| Abstract(Please copy/paste the abstract send to the congress) : |
SURVEY OF BIRDS (ANATIDAE: ANAS PLATYRHYNCHOS) FOR SCHISTOSOME PARASITES FROM NORTHERN IRAN
MAHDAVI Seifali (2), FARAHNAK Ali * (1), MOBEDI Iraj (1), MOLAEI RAD Mohammadbagher (1)
(1) Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
(2) Prevention and Control of Disease Center, Mazandaran University of Medical Sciences, Sari, Iran
*Corresponding author: Email: farahnak@tums.ac.ir
ABSTRACT
Background: The aim of the present study was to identify schistosome parasites in migratory birds (Anatidae: Anas platyrhynchos) which are the source of cercarial dermatitis disease in Mazandaran Province, Northern Iran.
Methods: A number of fresh mallards were bought from the markets of hunted birds. The respiratory tracts (nasal mucosa) and intestinal blood vessels were studied for adult worms. The nasal mucosa was separated, cleared and observed for egg and adult parasite by using a microscope. In order to separate the visceral schistosomes, after separating intestine, mesenteric vessels were studied under the lamp light, parasite samples were collected and described the morphological and morphometrical characteristics.
Results: Fifteen (13.6%) cases out of 110 studied birds had nasal mucosa contaminated with Trichobilharzia sp. egg. Besides that, two birds had adult worms of schistosome in mesenteric vessels i.e. Bilharziella polonica .
Conclusion: Cercaria of Trichobilharzia and Bilharziella parasites could be concerned as causative agents of cercarial dermatitis in aforementioned region.
Keywords: Trichobilharzia sp., Bilharziella polonica, Anas platyrhynchos, Parasite, Iran
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COMPARATIVE EVALUATION OF IN VITRO EFFECTS OF PRAZIQUANTEL (PZQ) ON THE ENZYME ACTIVITIES OF THE EXCRETORY - SECRETORY PRODUCTS (ESP) OF FASCIOLA HEPATICA AND F. GIGANTICA PARASITES
FARAHNAK Ali* (1), GOLMOHAMADI Taghi (2), ESHARGHIAN Mohammadreza (3), FARAHNAK Sepideh (4)
(1) Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
(2) Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
(3) Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
(4) Dept. of Medical Engineering, Azad University, Tehran, Iran
*Farahnak@tums.ac.ir
Abstract
Introduction:
Excretory-secretory products (ESP) play an important role in the host biochemical defense by means of activities of detoxifying and antioxidant enzymes. The aim of this study was to evaluate PZQ (anthelmintic drug) effect by detection of glutathione S- transferase (GST) and superoxide dismutase (SOD) enzymes activities in ESP samples of Fasciola hepatica and F. gigantica.
Materials and Methods:
F.gigantica and F. hepatica adult parasites were collected alive from sheep liver tissues, washed and cultured within buffer Media. Treated ESP samples by PZQ and control samples for each species were collected, centrifuged and supernatants were stored at -20C. SOD and GST enzymes activities of ESP samples were measured by using spectrophotometer. To determine the difference between ESP of treated and control samples, statistical test was conducted.
Results:
Findings analysis revealed GST and SOD enzyme activities in treated ESP samples of Fasciola gigantica significantly higher than F. hepatica (p < 0.05).
Conclusion:
Based on the results of present work, F. hepatica has less capability to protect against PZQ than F.gigatica.
Key Words: Fasciola gigantica; F. hepatica; Excretory-Secretory Products; Praziquantel; Superoxide dismutase; Glutathione S- transferase, Parasite |
| Keywords of your Abstract : |
Keywords: Trichobilharzia sp., Bilharziella polonica, Anas platyrhynchos, Parasite, Iran
and
Key Words: Fasciola gigantica; F. hepatica; Excretory-Secretory Products; Praziquantel; Superoxide dismutase; Glutathione S- transferase, Parasite |