Tehran University of Medical Sciences
Office of Vice-Chancellor for Global Strategies & International Affairs
International Human Capacity Development (IHCD)
Code : 9345-350756      Publish Date : Monday, January 4, 2016 Visit : 1352

Intl. Congress form | International Congress Report | International Congress Report For Faculty | BASAL LIFE SCIENCE WEEK

BASAL LIFE SCIENCE WEEK
BASAL LIFE SCIENCE WEEK
 
Application Code :
306-0215-0128
 
Created Date : Wednesday, October 7, 2015 11:01:06Update Date : Tuesday, December 15, 2015 09:41:25
IP Address : 194.225.51.101Submit Date : Tuesday, December 15, 2015 09:42:19Email : pasbakhsh@hotmail.com
Personal Information
Name : Parichehr
Surname : Pasbakhsh
School/Research center : School of Medicine
If you choose other, please name your Research center :  
Position : Professor
Tel : +98-21-44644585
Information of Congress
Title of the Congress : BASAL LIFE SCIENCE WEEK
Title of your Abstract : Comparison of differentiation potential of male mouse adipose tissue and bone marrow derived-mesenchymal stem cells into germ cells
Destination Country : Switzerland
From : Monday, September 21, 2015
To : Friday, September 25, 2015
Abstract(Please copy/paste the abstract send to the congress) : Background: Recent publications about differentiation of stem cells to germ cells have motivated researchers to make new approaches to infertility. In vitro production of germ cells improves understanding differentiation process of male and female germ cells. Due to the problem of using embryonic stem cells (ESC), it’s necessary the mentioned cells be replaced with some adult multi-potent stem cells in laboratories. 
Objective: The aim of this study was to obtain germ cells from appropriate source beyond ESC and compare differential potentials of adipocytes derived stem cells (ADMSCs) with bone marrow derived stem cells (BMMSCs).
Materials and Methods: To find multi-potential entity, after providing purified ADMSCs and BMMSCs, differentiation to osteoblast and adipocyte was confirmed by using appropriate culture medium. To confirm mesenchymal lineage production superficial markers (expression of CD90 and CD44 and non-expression of CD45 and CD31) were investigated by flowcytometry. Then the cells were differentiated to germ cells in inductive medium containing retinoic acid for 7days. To evaluate germ cells characteristic markers [Dazl (Deleted in azoospermia-like), Mvh (Mouse vasa homolog gene), Stra8 (Stimulated by retinoic acid) and Scp3 (Synaptonemal complex protein 3)] flowcytometry, imunoflorescence and real time PCR were used.
Results: Both types of cells were able to differentiate into osteoblast and adipocyte cells and presentation of stem cell superficial markers (CD90, CD44) and absence of endothelial and blood cell markers (CD31, CD45) were confirmative The flowcytometry, imunoflorescence and real time PCR results showed remarkable expression of germ cells characteristic markers (Mvh, Dazl, Stra8, and Scp3).
Conclusion: It was found that although ADMSCs were attained easier and also cultured and differentiated rapidly, germ cell markers were expressed in BMMSCs significantly more than ADMSCs.
Keywords of your Abstract : Key Words: Mesenchymal stem cells, Infertility, Germ cells, Retinoic acid
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/11925/Forms/306/BLSW 2015.docxparichehr acceptance_1.pdf
The presentation : Poster
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/11925/Forms/306/programme-blsw2015[1]_1.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/11925/Forms/306/خلاصه مقاله_2.pdf
Where has your abstract been indexed? : none
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : 3000
Delegates from which countries presented in the congress? : FROM OVER 50 COUNTRIES SWITZERLAND, USA, UNITED KINGDOM.............
Were the delegates of any other organizations present in the congress? : No
If yes, please write the names of the organizations in the box :  
What were the responses to your talking points? Were specific questions or concerns raised? : Good.Some questions were raised about : the findings of this study and the material and method of my experiance...The congress was scheduled for 3 days . My poster presentation was presented ..Wednesday 23 September 2015.
 
If you met staff members, please list their full names & positions. : .
Please inform us if there are any follow up actions we need to talk with the members of the congress : No special thing to mention. The congress was scheduled for 3 days where recent developments in labratories were exhibited.That was a good time for labs to find the best and newest labratory equipments................................................................................................................................................................................................................................................................



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Your experiences about the travel processes(Providing ticket, accommodation,...) : The financial suport of Tehran university of medical sciences is not enough for providing ticket ,registration and accomodation
Please give a briefing of your own observations and outcomes of the congress: : There were over 100 scientific talks,200 poster presentations.10 industry symposia and more than 100 exhibitors .covering a wide array of topic touching every facet of basic and applied research activities.it was exciting time for life science research ,as many fundamental discoveries in laboratories around the world had transitioned into break through applications for human health.scientific forums were in tittle of peptide therapeutics , digital health care,next generation sequencing,infectious diseases,......during symposium you will learn how the latest advances in their confocal high-content imaging technollogies will help...... .many fundamental discoveries in labratories around the world were introduce by exhibitors. Over 100 companies present their products and services to industry leaders and decision makers.the vision, the knowledge, the ability and the experience of the participant will help us to improve our knowledge and way into the future