Tehran University of Medical Sciences
Office of Vice-Chancellor for Global Strategies & International Affairs
International Human Capacity Development (IHCD)
Code : 9822-350909      Publish Date : Sunday, February 7, 2016 Visit : 2044

Intl. Congress form | International Congress Report | International Congress Report For Students and Staff | ISSCR2015

ISSCR2015
The report of ISSCR2015 by Parisa Shabani
Application Code :
306-0215-0154
 
Created Date : Saturday, September 19, 2015 09:10:48Update Date : Monday, January 11, 2016 16:11:01
IP Address : 217.146.218.171Submit Date : Monday, January 11, 2016 16:11:13Email : parisa83j@gmail.com
Personal Information
Name : Parisa
Surname : Shabani
School/Research center : School of Medicine
If you choose other, please name your Research center :  
Position : student
Tel : +98-21-88915818
Information of Congress
Title of the Congress : ISSCR2015
Title of your Abstract : Eicosapentaenoic acid enhances differentiation of embryonic stem cell into cardiomyocyte
Destination Country : Sweden
From : Wednesday, June 24, 2015
To : Saturday, June 27, 2015
Abstract(Please copy/paste the abstract send to the congress) : Eicosapentaenoic acid enhances differentiation of embryonic stem cell into cardiomyocyte
Parisa Shabani1,2, Hossein Baharvand2, Naser Aghdami2, Mahmood Doosti1
1. Department of Clinical Biochemistry, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2.Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
Embryonic stem cells (ESCs) offer a reliable means to produce acceptable number of functional cardiomyocytes to exploit in cell therapy, however low efficiency of differentiation hampers its therapeutic use. Although a vast number of chemical compounds have been tested on efficiency of cardiac differentiation, the effect of fish oil components like eicosapentaenoic acid (EPA) remained unstudied. EPA has been reported to have several cardioprotective effects, but there is no study about its contribution in cardiac differentiation. In the present study, mouse ESCs were induced to differentiate using hanging drops to form embryoid bodies and treatment with ascorbic acid, and in order to examine the effect of EPA, they were treated with different concentration of EPA simultaneously. Gene and protein expression and functional properties of cardiomyocytes derived from ESCs were evaluated. EPA at low concentration increased percentage of beating and beating area. It also upregulated mRNA expression of Nkx2.5, Mef2c, α-MHC, cTnT and CX43 significantly. Flowcytometric analysis showed that the percentage of α-MHC positive cells in EPA-treated group was higher than control group. However, these findings have not been observed at higher concentrations of EPA. In conclusion, we have demonstrated that treatment of mESCs undergoing cardiac differentiation with low concentration of EPA enhanced cardiac differentiation and exhibited synergistic effects with ascorbic acid.
Keywords of your Abstract : Eicosapentaenoic acid, Differentiation, Embryonic stem cell, Cardiomyocyte
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/26493/Forms/306/acceptance.pdf
The presentation : Poster
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/26493/Forms/306/cover_of_abstract.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/26493/Forms/306/abstract.pdf
Where has your abstract been indexed? : ISI
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : I do not know exactly
Delegates from which countries presented in the congress? : All over the world, from America, Germany, Japan, Switzerland, Poland
Were the delegates of any other organizations present in the congress? : No
If yes, please write the names of the organizations in the box :  
What were the responses to your talking points? Were specific questions or concerns raised? : One of the specific questions was regarding applying crisper/cas9 system in order to modify stem cells genetically prior to cell therapy
If you met staff members, please list their full names & positions. : Gordon M. Keller, PhD
McEwen Centre for Regenerative Medicine, University Health Network, Canada
Christine L. Mummery, PhD
Leiden University Medical Centre, The Netherlands
Please inform us if there are any follow up actions we need to talk with the members of the congress : There is not any follow up actions you need to talk with the members of the congress, but if you have more question you could ask Nathan L Chamberlain, l Manager, Membership and Meeting Services, International Society for Stem Cell, Research, 5215 Old Orchard Rd, Ste, 270 l Skokie, IL 60077 USA
Main: +1 224-592-5700 l, Direct: +1 224-592-5756
Your experiences about the travel processes(Providing ticket, accommodation,...) : It was not difficult to buy ticket , but since I did not have credit card, paying for hotel reservation, membership in ISSCR and registeration was difficult
Please give a briefing of your own observations and outcomes of the congress: : ISSCR provided me an opportunity to share my own data, learn from peers, and discover global advances within the stem cell field. The topics included regeneration and engraftment, disease modeling, therapy with stem cells, pluripotency and mechanisms of reprogramming, immunology and stem cells, making tissues and organs. I chose to attend the presentations regarding pluripotency and differention into different cell types espicially cardiomyocyte. I had also the chance to meet and talk to well-known professors in stem cell.