Tehran University of Medical Sciences
Office of Vice-Chancellor for Global Strategies & International Affairs
International Human Capacity Development (IHCD)
Code : 9822-351892      Publish Date : Wednesday, October 5, 2016 Visit : 1865

Intl. Congress form | International Congress Report | International Congress Report For Students and Staff | 3rd. international conference on science and engineering

3rd. international conference on science and engineering
The report of 3rd. international conference on science and engineering by Majid Rahmati
Application Code :
306-0216-0099
 
Created Date : Thursday, June 30, 2016-02:59 02:59:23Update Date : Wednesday, September 21, 2016-11:17 11:17:28
IP Address : 89.45.56.40Submit Date : Wednesday, September 21, 2016-11:18 11:18:10Email : rahmatim@razi.tums.ac.ir
Personal Information
Name : Majid
Surname : Rahmati
School/Research center : School of Advanced Technology in Medicine
If you choose other, please name your Research center :  
Position : student
Tel : +98-23-32247825
Information of Congress
Title of the Congress : 3rd. international conference on science and engineering
Title of your Abstract : Cloning and expression of human bone morphogenetic protein-2 gene in Leishmania tarentolae
Destination Country : Turkey
From : Thursday, June 2, 2016
To : Thursday, June 2, 2016
Abstract(Please copy/paste the abstract send to the congress) : Several expression hosts have been employed for the production of bone morphogenetic protein-2 (BMP- 2), inducing de novo bone formation and is widely used in clinical applications. However, every expression system has its merits and demerits. For example, although Escherichia coli is simple and robust, but inept to make disulfide bond; hence, heterologous proteins are produced in the form of inclusion bodies, entailing in vitro refolding strategies. On the other hand, yeast, which
is a lower eukaryote, is able to perform glycosylation of the recombinant proteins. However, its glycosylation pattern is different from human beings. Ultimately, Leishmania tarentolae, a parasite protozoan of the lizard, unifying both the characteristics of prokaryotes and eukaryotes, has been
introduced for the expression of glycoproteins. It is capable of making disulfide bonds, expressing correctly folded and biologically active recombinant proteins. In the current study, we successfully cloned and expressed proBMP-2 gene in L. tarentolae. Integration of the gene was confirmed by
PCR; whereas, transcription of the coding gene was verified by back translating the target mRNA.
Besides, the expression of BMP-2 was assessed by SDS-PAGE and Western blotting. A 12 kDa band was obtained, equivalent to monomeric form of BMP-2.
Keywords of your Abstract : Bone morphogenetic protein 2, Morphogenesis, Growth differentiation factor 2, Osteogenesis, Spinal cord injuries, Glycosylation.
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/31207/Forms/306/certificate_1.pdf
The presentation : Oral
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/31207/Forms/306/cover.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/31207/Forms/306/Abstract_Turkey.pdf
Where has your abstract been indexed? : ISI
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : About 200 persons
Delegates from which countries presented in the congress? : Iran, Italy, Iraq,Turkey,India,Pakistan,Spain, UK and etc.
Were the delegates of any other organizations present in the congress? : Yes
If yes, please write the names of the organizations in the box : Australian Universal Academy,
What were the responses to your talking points? Were specific questions or concerns raised? : Congress attendees asked some questions regarding protein concentration, product yield, future directions and perspective of the study and possible suggestions. They also asked some technical questions concerning cloning approach and why I used this type of leishmania.
If you met staff members, please list their full names & positions. : Prof. Piero Formica the chairman of the congress
Please inform us if there are any follow up actions we need to talk with the members of the congress : I had some talk with scientists in the congress concerning our future collaborations. And they stated that we are always welcome in this regards. So, I think that it would be good idea and opportunity for our university to establish a connection for further early future collaborations. It not only help us to design international joint projects but also it could be an inception for our exchange programs.
Your experiences about the travel processes(Providing ticket, accommodation,...) : I book my accommodation personally once designated hotels by congress organizers were very expensive. I also purchased my ticket by myself.
Please give a briefing of your own observations and outcomes of the congress: : Once it was my first experience attending in an international congress out of Iran, it was really interesting for me. Moreover, it was a good opportunity for me to converse with colleagues and faculty of the many countries around the world. I also enjoy talking to some prominent scientists concerning my research projects and possible future collaborations. Finally, I also learnt how we can organize a congress in terms of organization, programs and types of presentations, accommodation and required physical space.