Tehran University of Medical Sciences
Office of Vice-Chancellor for Global Strategies & International Affairs
International Human Capacity Development (IHCD)
Code : 9822-352360      Publish Date : Saturday, February 4, 2017 Visit : 2372

Intl. Congress form | International Congress Report | International Congress Report For Students and Staff | TERMIS-UE 2016

TERMIS-UE 2016
The report of TERMIS-UE 2016 by. Nastaran Sabetkish
Application Code :
306-0216-0090
 
Created Date : Thursday, August 18, 2016-22:54 22:54:54Update Date : Wednesday, January 4, 2017-13:28 13:28:34
IP Address : 194.225.212.150Submit Date : Thursday, August 18, 2016-23:55 23:55:21Email :nastaran.sk@outlook.com
Personal Information
Name : Nastaran
Surname : Sabetkish
School/Research center : Pediatric Urology Research Center (PURC)
If you choose other, please name your Research center :  
Position : staff
Tel : +98-21-66565400
Information of Congress
Title of the Congress : TERMIS-UE 2016
Title of your Abstract : In-vivo human corpus cavernosum regeneration: fabrication of tissue-engineered corpus cavernosum in rat using the body as a natural bioreactor
Destination Country : Sweden
From : Tuesday, June 28, 2016
To : Friday, July 1, 2016
Abstract(Please copy/paste the abstract send to the congress) : INTRODUCTION: Few researches have been conducted to develop an ideal method for tissue-engineering of corpus covernosum [1-2]. We produce a decellularized human corpus cavernosum scaffold and investigate the in-vivo cell seeding of the scaffold after transplantation into the rat omentum.
METHODS: Eight adult human male corpus cavernosum were obtained in sterile condition after obtaining ethical committee approval. After dissecting the urethra, corpus cavernosum was decellularized by inserting an 18 gauge needle into the body of the tissue. The gauge was connected to a peristaltic pump to circulate the detergents in the corpus. Histopathological examinations, DNA quantification, scanning electron microscopy (SEM), and tensile test were performed to evaluate the efficacy of decellularization and extracellular matrix (ECM) preservation. A section of decellularized scaffold was washed several times and transplanted into the omentum of 4 male healthy Sprague Dawley rats and located into the scrotum. Biopsies were taken 1, 3 and 6 months after transplantation. Histological examination, SEM, DNA quantification, and immunohistochemical (IHC) staining were performed to assess the efficacy of natural recellularization 

Fig 1: Surgical technique of decellularized human corpus covernosum transplantation 
RESULTS: The results of the examinations performed prior to transplantation, revealed a decellularized ECM resembling to the native tissue with normal pits that may be appropriate for further in-vivo cell seeding. Histopathology examination of the biopsies after transplantations confirmed successful cell seeding with endothelium-like cells in different time-points. CD 34 staining was dominant in the short-time biopsies, while CD31 staining was higher than CD34 in long-term specimens.

Fig 2: Histological evaluations and SEM analysis of decellularized scaffolds 
DISCUSSION & CONCLUSIONS: This study theoretically may pave the road for corpus covernosum regeneration by transplantation of decellularized scaffolds into the scrotum. The feasibility of natural bioreactor in recellularizing corpus covernosum was confirmed. This technique may have the potential to facilitate homologous transplantation for repair of corpus defects.
REFERENCES: 1 C Ji, F Min, W Liang, et al (2011) BJU Int 107: 1638-46. 2 G An, C Ji, Z Wei, et al (2013) Urol 81: 424-31.
DISCLOSURE: Authors have nothing to disclose.
Keywords of your Abstract : corpus, tissue-engineering, regenerative medicine
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/32195/Forms/306/Acceptance.pdf
The presentation : Poster
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/32195/Forms/306/Cover.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/32195/Forms/306/Abstract.pdf
Where has your abstract been indexed? : ISI
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : 4
Delegates from which countries presented in the congress? : Italy- France- Turkey- Spain- Japan- Russia
Were the delegates of any other organizations present in the congress? : Yes
If yes, please write the names of the organizations in the box : Bioreactor Company
What were the responses to your talking points? Were specific questions or concerns raised? : As our posters, presentations and the framework of our studies were brief and feasible, there were no specific questions or concerns raised.
If you met staff members, please list their full names & positions. : Co-chair:
Jöns Hilborn, May Griffith
Please inform us if there are any follow up actions we need to talk with the members of the congress : I should inform you that there are no follow up actions that need your talk with the members of the congress. We have received our certification from the tissue engineering and regenerative medicine society and I think that no further follow- up is needed. Our abstracts are also published in the abstract book
Your experiences about the travel processes(Providing ticket, accommodation,...) : We got lots of experience during the travel processes regarding the submission process of the abstracts, registration, buying tickets, reserving hotel
Please give a briefing of your own observations and outcomes of the congress: : As mentioned above, the congress was about tissue engineering and regenerative medicine and lots of presentation and posters were presented in the congress regarding the new approaches in cell culture, cell seeding and tissue regeneration both in in-vivo and in-vitro models. Additionally, new technologies such as bioreactors, cell seeding machines were presented by the organizers. as a result, we have got new ideas for our future studies in which we could apply these techniques for better results. we have also got access to the contact information of several companies with whom we can have further collaborations.