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Abstract(Please copy/paste the abstract send to the congress) : |
P-093 Inflammasome complex gene expression and its
relationship with infertility of male rats after spinal cord injury
M. Bazrafkan1
, B. Nikmehr2
, G. Hassanzadeh2
, A. Shahverdi3
,
M. Marzban4
, S. Kiani5
, H. Choobineh6
1
Tehran University of Medical Sciences- School of Medicine- -, department of
anatomy, tehran, Iran
2
Tehran University of Medical Sciences-School of Medicine, Department of Anatomy,
tehran, Iran
3
Royan Institute for Reproductive Biomedicine- ACECR, Department of Embryology
at Reproductive Biomedicine Research Center, tehran, Iran
4
Shiraz University of Medical Science-, Students research center committee, shiraz,
Iran
5
Royan Institute for Stem Cell Biology and Technology, Department of Stem Cells and
Developmental Biology- Cell Science Research Center, tehran, Iran
6
Tehran university of medical sciences-School of Allied Medical Sciences, Zoonosis
Research Center, tehran, Iran
Study question: Evaluation of Inflammasome genes expression and their
activity pattern in testis of spinal cord injury (SCI) rat models.
Summary answer: Testis is one of the sources of inflammasome genes which
induces pyroptosis and resulted in reduction of sperm concentration and motility
in SCI model.
What is known already: Many young men with spinal cord injury (SCI) suffer
from infertility. Previous studies showed the low sperm concentration, motility
and abnormal morphology in SCI infertile men. Some researchers suggested
immunologic reasons for diminished sperm quality in SCI patients. They identi-
fied some inflammasome complex productions such as caspase-1 in sperm of
these patients, but the class of upstream genes and the time of starting activity
in this complex was not determined clearly.
Study design, size, duration: Mature male rats were used in this study.
The rats were divided into four groups (n = 4): three surgery groups that SCI
at level of T10 performed on them and one intact group as control. We scari-
fied group one, a day after SCI surgery, group two, 3 days after and group 3 a
week after surgery. Testes were collected. The most known inflammasome
upstream genes expression includes NLRP1a, NLRP3, NLRC4, and AIM2
were assessed.
Participants/materials, setting, methods: Mature male wistar rats with
250-300 gr. body weight were used. The above- mentioned genes were examined
by qrt-PCR. The data analyzed by The Kruskal-Wallis Test and Games
Howel post hoc test to find the difference between various time points.
Main results and the role of chance: According to the statistical analysis
test the gene expression level in NLRP3 (χ2
= 11.373,df=3, P-Value .010) and
AIM2 (χ2
= 9.201,df=3, P-Value.027) showed significant difference (p < 0.05).
No significant difference was detected in NLRP1a and NLRC4 genes expression
between the test and control groups in any time points. In group 3 the expression
of NLRP3 was significantly different in compare to control group (MD=
-2.80, P =0.04) also in group one, the expression of AIM2 (MD= 1.73,
P = 0.025) was significantly up regulated in contrast to control group. For the
first time we find that expression of NLRP3 as the most known upstream gene
of inflammasome complex up-regulates one week after SCI in the testis of rat
model. Also we find that expression of AIM2 as another gene of infalmmasome
complex up-regulates a day after injury. These up-regulations indicate initiation
of inflammasome complex activity. The Inflammasome complex activity can
induce pyroptosis (a model of Programmed cell death after formation of inflammasome
complex). We suggest that pyroptosis can be the cause of low sperm
motility and count in SCI rat models.
Limitations, reasons for caution: The main limitations of this study could
be the low number of rats. Also, based on the ethical considerations in this
study we only examined the effects of SCI in rat model but not in human.
Wider implications of the findings: Knowing the exact genes of inflammasome
complex that express in testis after SCI and the exact time of
Abstracts of the 33rd Annual Meeting of ESHRE, Geneva, Switzerland 2 to 5 July 2017 i179
inflammasome up-regulation can be helpful for administration of anti inflammasome
drugs in exact time to suppress inflammasome complex formation, excessive
pyroptosis and also improve sperm count and motility.
Trial registration number: it is basic science. |