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Title of the Congress : |
12th International Conferene on Molecular Epidemiology and Evolutionary Genetics of Infectious Disease |
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Title of your Abstract : |
1- Adamantane & neuraminidase resistant influenza A/H3N2 isolated in Iran from 2005 to 2013
2- The detection of Oseltamivir-resistant influenza A/H3N2 viruses using a Real-time RT-PCR assay and comparison the results with direct sequencing
3- Pattern of HRSV epidemics in Iran: Molecular epidemiology of the G protein over seven years |
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country : |
Thailand |
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From : |
Thursday, December 11, 2014 |
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To : |
Saturday, December 13, 2014 |
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Abstract(Please copy/paste the abstract send to the congress) : |
1- Adamantane and neuraminidase resistant influenza A/H3N2 isolated in Iran from 2005 to
2013
J. Yavarian*, T. Mokhtari Azad, N.Z. Shafiei Jandaghi
Tehran University of Medical Sciences, Iran
Introduction: Influenza viruses are one of the most important etiological agents of respiratory
disease. Vaccination and antiviral treatments are the sole and essential ways for the prevention
and control of influenza infection. During an influenza epidemic before the production of
effective vaccine, antiviral treatments are the first step for the prevention and treatment of
infection. Adamantanes and neuraminidase inhibitors are influenza antiviral drugs. Because of
the increasing of drug resistant viruses, the aim of this study was the evaluation of the antiviral
drug resistance in influenza A/H3N2 viruses from 2005-2013 in Iran. Methods: In 44585
samples collected during nine years, 7447 (16.7%) were positive for influenza viruses which
1036(2.3%) were A/H3N2 viruses. In this study 50 influenza A/H3N2 viruses isolated in cell
culture during nine years were tested. All samples were subjected to M and NA gene
sequencing. After RNA extraction from cell culture supernatants, RT-PCR with one step RTPCR
kit was performed. The expected size of the PCR products was analyzed by
electrophoresis. The PCR products were sequenced for finding the drug resistant mutants.
Results: All influenza A/H3N2 viruses except four viruses circulating during 2005-2006 had
Ser31Asn mutation at M2 channel protein. In the analysis of neuraminidase gene none of the
A/H3N2 viruses had K292R, E119V and N294S mutations responsible for drug resistant strains.
Discussion: This study showed circulating A/H3N2 viruses were resistant to adamantanes but
susceptible to neuraminidase inhibitors. The national data analyzed in this research may help
increase knowledge about influenza virus antiviral drug resistance, which is a global public
health concern.
Keywords: Influenza A/H3N2, Drug resistant
2- The detection of oseltamivir-resistant influenza A/H3N2 viruses using a Real-time RTPCR
assay and comparison the results with direct sequencing
J. Yavarian*, T. Mokhtari Azad, M.H. Karbalaie Niya
School of Public Health, Tehran University of Medical Sciences, Iran
Introduction: Currently, there is an increasing risk of a worldwide influenza A virus epidemics,
then research about the prophylaxis and treatment of these viruses and detection of drug
resistant mutants is a must. Nowadays oseltamivir is the suitable drug for preventing and
treatment of these viruses. Drug resistant viruses have already detected by mutations of the
enzyme's active site in the neuraminidase (NA) gene that cause amino acid substitutions
predominantly at positions like 119,274,292. The objective of this research was designing of two
probes for simultaneous detection of oseltamivir resistant and sensitive A/H3N2 viruses by
Real-time RT-PCR assay and comparison the results with direct sequencing. Methods: In this
study Real-time RT-PCR assay was performed on influenza A/H3N2 NA gene then
conventional RT-PCR assay and nucleotide sequencing were performed towards confirmation
and evaluation of mutation site sequences. Results: Of 50 A/H3N2 specimens collected in
2012, all were negative for H274Y mutation by Real-time RT-PCR assay and the results were
the same with sequence analysis of the NA gene and also there were no R292K mutation in
these samples. Discussion: Although Real-time RT-PCR method may not be able to detect
new mutations but in laboratories only equipped with Real-time PCR machine, this technique
would be useful in detection of sensitivity or resistance to oseltamivir or other influenza
antivirals. Meanwhile quick and accurate recognition of drug resistant mutants is applicable for
effective treatment strategies then we suggest the use of Real-time RT-PCR assay as rapid and
highly practical monitoring test which could be performed for detection of point mutations.
Keywords: Influenza A/H3N2, Oseltamivir resistant
3-Pattern of HRSV epidemics in Iran: Molecular epidemiology of the G protein over seven
years
E. Faghihloo, J. Yavarian*, T. Mokhtari Azad
School of Public Health, Tehran University of Medical Sciences, Iran
Introduction: Human respiratory syncytial virus (HRSV) is the most important cause of lower
respiratory tract infection in infants. HRSV strains have different antigenic groups and have
been classified into broad subgroups, A and B. In order to have information on the molecular
epidemiology and genetic diversity of HRSV in Iran, we studied the genetic variability of both
group A and B HRSV strains during seven consecutive years by sequencing the hypervariable
C-terminal domain of G protein.
Methods: A total of 485 children < 2 years of age who were negative for influenza viruses, were
screened by using nested RT-PCR for the presence of HRSV in this research.
Results: HRSV was detected in 94 (19.38%) of the samples. Group A viruses were isolated
during each year, while group B viruses were isolated during 2009 and 2013. Phylogenetic
analysis showed that all HRSV group A viruses belonged to three genotypes: GA1, GA2, GA5
and the group B viruses were in BA genotype.
Discussion: This research was the first report of the long term study of genetic diversity of
HRSV G gene in Iran. Both A and B HRSV groups co-circulated in the children with A
predominancy. However further surveillance is required with more sample size per year for
detection of the variation and circulation pattern of HRSV. |
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Keywords of your Abstract : |
1- Keywords: Influenza A/H3N2, Drug resistant
2- Keywords: Influenza A/H3N2, Oseltamivir resistant
3- Keywords: HRSV, Molecular Epidemiology, Iran |
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Acceptance Letter : |
http://gsia.tums.ac.ir/images/UserFiles/23364/Forms/306/Acceptance_letters.rar |
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The presentation : |
Oral |
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The Cover of Abstract book : |
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Published abstract in the abstract book with the related code : |
http://gsia.tums.ac.ir/images/UserFiles/23364/Forms/306/Thailan_Abstracts.rar |
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Where has your abstract been indexed? : |
ISI |
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If you choose other, please name : |
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