Code : 9345-350518      Created Date : Sunday, November 1, 2015   Update Date : Sunday, November 1, 2015    Visit : 1683

XXIV International Symposium on Morphological Sciences

The report of XXIV International Symposium on Morphological Sciences by Dr. Tayebeh Rastegar
Application Code :
306-0215-0099
 
Created Date : Monday, September 14, 2015 14:43:17Update Date : Wednesday, October 28, 2015 10:03:02
IP Address : 194.225.51.101Submit Date : Wednesday, October 28, 2015 10:03:24Email : trastegar@tums.ac.ir
Personal Information
Name : tayebeh
Surname : rasteagr
School/Research center : School of Medicine
If you choose other, please name your Research center :  
Position : Assistant professor
Tel : +98-21-64053339
Information of Congress
Title of the Congress : XXIV International Symposium on Morphological Sciences
Title of your Abstract : Differentiation of Bone Marrow Mesenchymal Stem Cells to Germ Cells by Sertoli Cells Conditioned Medium
Country : Turkey
From : Wednesday, September 2, 2015
To : Sunday, September 6, 2015
Abstract(Please copy/paste the abstract send to the congress) : Differentiation of bone marrow mesenchymal stem cells to germ cells by Sertoli cells conditioned medium 
Rastegar T*, Hajian M, Baazm M 
Department of Anatomical Sciences,school of Medicine, tehran university of Medical Sciences,Tehran, Iran
Sertoli cells are responsible for differentiation of germ stem cells to spermatozoa by secreting some factors in vivo. Mesenchymal stem cells could differentiate into germ cells under appropriate conditions. This study investigated differentiation of bone marrow derived mesenchymal stem cells (BMSCs) to germ line cells using Sertoli cells conditioned medium. Mesenchymal stem cells were isolated from the bone marrow of adult male mice and cultured for 7 days until 80% confluence. On the other hand, Sertoli cells were enriched using lectin coated plates, cultured for 48 hours and collected medium. Mesenchymal stem cells cultured using Sertoli cells conditioned medium. We have found that conditioned medium prepared from adult sertoli cells supports the differentiation of BMSCs into putative germ cells. BMSCs cultured with Sertoli cells condition medium, expression of meiotic and post-meiotic genes was observed (Mvh, ID4,Piwil2) by RT-PCR and protein expression of germ-cell-specific marker Scp3 identified by immunocytochemistry. Gel Electrophoresis of PCR showed expression Mvh, ID4, Piwil2 transcripts in BMSCs differentiated to germ like cells while expression of these molecular marker didn’t observed in control cells (p≤0.05) . Sertoli cells conditioned medium may allow induction of BMSCs to germ cells for treatment of male infertility. 
Keywords of your Abstract : Bone marrow mesenchymal stem cells, Sertoli cell conditioned medium, Differentiation, germ cell
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/26391/Forms/306/acceptance_2.pdf
The presentation : Oral
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/26391/Forms/306/جلد_2.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/26391/Forms/306/خلاصه_2.pdf
Where has your abstract been indexed? : none
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : 500
Delegates from which countries presented in the congress? : kenia- USA- Austrailia- nigeria.......
Were the delegates of any other organizations present in the congress? : No
If yes, please write the names of the organizations in the box :  
What were the responses to your talking points? Were specific questions or concerns raised? : Testicular-cell-conditioned medium were induced human umbilical mesenchyme cells (hUMSCs) to differentiate into germ cells?

Sertoli cell condition medium can significantly differentiates human emberyonic stem cells (hESC) lines towards the germ cell lineage?

Conditioned medium collected from testicular cell cultures developed embryonic stem cells into ovarian structures containing oocytes?

Testicular somatic cells culture has become an important and innovative tool for applied research in reproductive biology?
If you met staff members, please list their full names & positions. : Paxinus peter
Please inform us if there are any follow up actions we need to talk with the members of the congress : It is necessary for mother’s university of Iran has been some equipment. We don’t even have equipment like Kermanshah University of medical sciences in our lab of cadaver. We need have plastination section of cadavers for studying student in graduate and postgraduate students. Please talk about teaching of english to members involved in congress. Take better food and tours.
Your experiences about the travel processes(Providing ticket, accommodation,...) : We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students. For 4 days faculty of anatomy staff of Anatomy group and postgraduate students. It was the first scientific trip for our students, so they know how to prepare in congress, how to present, how to meet scientific and what we need to learn for using in research and techniques. Most of the group members had oral presentation and rest poster presentation. in my opinion all of them were excellent and we had a good feedback from the persons who were in sessions.We are the greatest group in this congress. Most of accompanying had oral presentation and a best scientific trip for our students.
Please give a briefing of your own observations and outcomes of the congress: : it's good for me. I met some important person that involved in Anatomy teaching, Stem cells, Antropology and Stereiology.We meet scientific members like Paxinus and.....Most of the group members had oral presentation and rest poster presentation. in my opinion all of them were excellent and we had a good feedback from the persons who were in sessions.Postgraduate students, know how to prepare in congress, how to present, how to meet scientific and what we need to learn for using in research and techniques. In my opinion all of them were excellent and we had a good feedback from the persons who were in sessions.

 

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