Code : 9822-352214      Created Date : Wednesday, December 28, 2016   Visit : 2544

Tissue Engineering ad Regenerative Medicine International Society (TERMIS-EU Meeting)

The report of Tissue Engineering ad Regenerative Medicine International Society (TERMIS-EU Meeting) by Shabnam Sabetkish
Application Code :
306-0216-0089
 
Created Date : Thursday, August 18, 2016-21:27 21:27:52Update Date : Monday, November 21, 2016-22:43 22:43:02
IP Address : 151.242.104.193Submit Date : Monday, November 21, 2016-22:43 22:43:25Email :Shabnamita.sk@gmail.com
Personal Information
Name : Shabnam
Surname : Sabetkish
School/Research center : Pediatric Urology Research Center (PURC)
If you choose other, please name your Research center :  
Position : staff
Tel : +98-21-66565400
Information of Congress
Title of the Congress : Tissue Engineering ad Regenerative Medicine International Society (TERMIS-EU Meeting)
Title of your Abstract : Oral presentation: Future prospects for human tissue engineered urethra transplantation: decellularization and recellularization-based urethra regeneration 
 
Destination Country : Sweden
From : Tuesday, June 28, 2016
To : Friday, July 1, 2016
Abstract(Please copy/paste the abstract send to the congress) : INTRODUCTION: The role of tissue-engineered urethra as a potential substitute for effective treatment of urethral strictures and anomalies has been previously discussed [1-2]. In the current study, we evaluated the histological characteristics of decellularized human urethra after transplantation into the rat omentum.
METHODS: Eight adult human male urethras accompanied with the surrounding corpus spongiosum were obtained in sterile condition after obtaining ethical committee approval. Then, the tissues were decellularized with detergent-based method. The efficacy of decellularization and extracellular matrix (ECM) preservation was evaluated by histopathological examinations, DNA quantification, scanning electron microscopy (SEM), and tensile test. Decellularized scaffolds were washed to remove the detergents and transplanted into the omentum of 4 male healthy Sprague Dawley rats and located into the scrotum. Biopsies were taken 1, 3, and 6 months postoperatively to assess the natural recellularization. Histological examination, SEM, DNA quantification, and immunohistochemical (IHC) staining were performed.
RESULTS: DNA quantification and histological evaluations confirmed the removal of all cellular and nuclear components in urethral and surrounded corpus spongiosum scaffolds. Tensile test confirmed that biomechanical properties of the urethral ECM were well preserved. In-vivo recellularization revealed promising results in cell seeding of epithelium-like cells in the lining of the urethra as well as smooth muscle cells in the wall structure. Progressive angiogenesis was also detected in the transplanted scaffolds during the time-points while inflammatory markers were negative. 
DISCUSSION & CONCLUSIONS: The results suggest that we can prospect to perform urethral reconstruction by using the body as a natural bioreactor that can paw the road for clinical application of acellular matrix in urological reconstructive surgery.
Keywords of your Abstract : Urethra, Tissue engineering, Scaffold
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/26984/Forms/306/Acceptance letter_3.pdf
The presentation : Oral
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/26984/Forms/306/Cover book_3.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/26984/Forms/306/Abstract_3.pdf
Where has your abstract been indexed? : ISI
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : 3
Delegates from which countries presented in the congress? : Italy, Spain, France, Turkey, Germany, Russia
Were the delegates of any other organizations present in the congress? : Yes
If yes, please write the names of the organizations in the box : Bioreactor company
What were the responses to your talking points? Were specific questions or concerns raised? : As the presentation and the study was brief and feasible for clinical application, there were no specific questions or concerns raised
If you met staff members, please list their full names & positions. : Co-chair: Jöns Hilborn and Co-chair: May Griffith
Please inform us if there are any follow up actions we need to talk with the members of the congress : I should inform you that there are no follow up actions you need to talk with the members of the congress as we have already received our certification from European Chapter Meeting of the Tissue Engineering and Regenerative Medicine International Society congress. I think that there is no need for any follow up action.Thank you so much for your kind consideration.
Your experiences about the travel processes(Providing ticket, accommodation,...) : We got lots of experiences during the travel. The hotel was reserved via booking.
Please give a briefing of your own observations and outcomes of the congress: : We learned more about pediatric urology diseases and the outcome was good. We met many pioneer professors in pediatric urology diseases. I gained invaluable experiences in the TERMIS Congress, on 28 June - 1 July, 2016 Uppsala, Sweden. It was a great pleasure for me to meet famous pediatric urologists and to evaluate my ability to present my work in an international congress outside my country. I decided to present my work in an international congress, it was difficult and stressful at first but of course that was worthy.

 

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