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Abstract(Please copy/paste the abstract send to the congress) : |
Macracanthorhynchus ingens in long-eared hedgehog (Hemiechinus auritus): Histopathology and
molecular characterization of the isolate
Sanaz Jafarpoor Azami1, Eshrat Beigom Kia,1, Iraj Mobedi1, Zahra Heidari1, Meysam Sharifdini2, Kourosh
Arzamani3, Bahareh Kamranrashani1
1Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences,
Tehran, Iran, 2Department of Medical Microbiology, School of Medicine, Guilan University of Medical Sciences,
Rasht, Iran, 3Vector-borne Diseases Research Center, North Khorasan University of Medical Sciences, Bojnurd,
Iran
Introduction: Macracanthorhynchus ingens belongs to perforated groups of acanthocephalan parasites which
are usually parasite of raccoon, but they can also infect human and animals. This is the first report of
Macracanthorhynchus ingens from long-eared hedgehog (Hemiechinus auritus) in Iran.
Methods: During examination of a hedgehog from Jajarm City in North Khorasan, Iran, nine acanthocephalan
helminthes were found in small intestine. Helminthological clearing and staining techniques were applied and
tissue sections prepared. For molecular analysis, after DNA extraction using designed primers for 18s ribosomal
RNA, PCR amplification and sequencing were performed.
Results: Tissue sections revealed the penetration of proboscis in the intestine. The sequences showed 100%
similarity with the sequences of Macracanthorhynchus ingens deposited in GenBank.
Conclusion: Occurrence of Macracanthorhynchus ingens in hedgehog in Iran constitutes new host species. |