|
Title of the Congress : |
9th european congress of tropical medicine and international health |
|
Title of your Abstract : |
Evaluation of antifungal activities of Bifidobacterium bifidum and Lactobacillus fermentum against toxigenic Aspergillus parasiticus |
|
Destination Country : |
Switzerland |
|
From : |
Sunday, September 6, 2015 |
|
To : |
Thursday, September 10, 2015 |
|
Abstract(Please copy/paste the abstract send to the congress) : |
Evaluation of antifungal activities of Bifidobacterium bifidum and Lactobacillus fermentum against toxigenic Aspergillus parasiticus
Roshanak Daie Ghazvini 1*; Ebrahim Koohsari 1; Farhad Niknejad 2; Ensieh Zibafar 1; Seyed Jamal Hashemi 1
1. Dept. of Medical Parasitology & Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.
2. Dept. of Laboratory Sciences and Medical Mycology & Parasitology, Golestan University of Medical Science Gorgan , Iran
*Corresponding Author: Tel: +98-21- 88951583 - E-mail: roshanak1043@yahoo.com
rdaie@tums.ac.ir
Background: Aflatoxins are one of the most important global concerns, especially in developing countries. They cause a wide spectrum of serious medical problems in human and animals and also economics losses.
Aims:In this study, it was evaluated the effect of Bifidobacterium bifidum PTCC 1644 and Lactobacillus fermentum PTCC 1744 isolates on the fungus growth rate and aflatoxin production in toxigenic Aspergillus parasiticus.
Methods: Mycelial growth inhibition of toxigenic Aspergillus parasiticus in the presence of Bifidobacterium bifidum and Lactobacillus fermentum was investigated by pour plate technique and weighting method of mycelial mass. Reduction of aflatoxin was evaluated in yeast extract sucrose broth (YESB) at 30C after seven days of incubation using HPLC method. Also, reduction of aflatoxin rate was observed in the presence of the metabolites of lactic acid bacteria by HPLC method. The data were analyzed by SPSS 21.
Results: The presence of the Bifidobacterium bifidum and Lactobacillus fermentum were affected significantly on the growth rate of Aspergillus parasiticus in comparison with controls without lactic acid bacteria (P≤0.05). Percentage of reductions in total aflatoxin and B1, B2, G1, G2 fractions by Bifidobacterium bifidum and Lactobacillus fermentum were more than 99%. Furthermore, the percentage of reduction of standard aflatoxin B1, B2, G1, G2 fractions by Metabolites produced of lactic acid bacteria were approximately 88.8% to 99.8% (p≤0.05).
Conclusion: Bifidobacterium bifidum and Lactobacillus fermentum can be employed as good biocontrol agents against growth and aflatoxin production by aflatoxigenic Aspergillus species.
Keywords: Aflatoxin, Bifidobacterium bifidum, Lactobacillus fermentum, Aspergillus parasiticus, HPLC
Aflatoxin, Bifidobacterium bifidum, Lactobacillus fermentum, Aspergillus parasiticus, HPLC |
|
Keywords of your Abstract : |
Aflatoxin, Bifidobacterium bifidum, Lactobacillus fermentum, Aspergillus parasiticus, HPLC |
|
Acceptance Letter : |
http://gsia.tums.ac.ir/images/UserFiles/21814/Forms/306/On_Tuesday.pdf |
|
The presentation : |
Poster |
|
The Cover of Abstract book : |
http://gsia.tums.ac.ir/images/UserFiles/21814/Forms/306/You_have_free_access_to_this_content.pdf |
|
Published abstract in the abstract book with the related code : |
http://gsia.tums.ac.ir/images/UserFiles/21814/Forms/306/ABS BOOK.PDF_1.pdf |
|
Where has your abstract been indexed? : |
ISI |
|
If you choose other, please name : |
|