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Code : 9345-352494 Created Date : Saturday, March 4, 2017 Visit : 1485

The report of 66. Kongress der DGMKG in Hamburg by Dr. Naghmeh Bahrami

Application Code :

306-0116-0068

Created Date : Saturday, June 18, 2016-14:56 14:56:24Update Date : Saturday, February 4, 2017-15:09 15:09:03
IP Address : 192.168.89.10Submit Date : Saturday, February 4, 2017-15:09 15:09:23Email : n-bahrami@sina.tums.ac.ir

Personal Information

Name :	naghmeh
Surname :	bahrami
School/Research center :	School of Dentistry
If you choose other, please name your Research center :
Position :	Assistant professor
Tel :	+98-21-42794304

Information of Congress

Title of the Congress :	66. Kongress der DGMKG in Hamburg
Title of your Abstract :	The effect of purmorphamine on differentiation of endometrial stem cells into osteoblast like cells on collagen-hydroxyapatite scaffolds
Destination Country :	Germany
From :	Wednesday, June 1, 2016
To :	Saturday, June 4, 2016
Abstract(Please copy/paste the abstract send to the congress) :	The main goal of periodontal treatment is to achieve regeneration with reconstruction of all the suitable components including periodontal ligament, alveolar bone, root cementum and connective tissue. The goal of this study was to assess the effect of purmorphamine (PMA) along with Collagen-Hydroxyapatite scaffold in inducing osteogenesis of endometrial stem cells (hEnSCs) in 2D and 3D culture media. The cells were cultured in an osteogenic differentiating media containing PMA in tissue culture plate (TCP as 2D group) and Collagen-Hydroxyapatite Scaffold (as 3D group) for 21 days. Structure of Collagen-Hydroxyapatite scaffold, cell morphology and cell attachment were analyzed with SEM and DAPI staining. The viability of cells on scaffold and osteoblast cells markers were assayed with MTT assay and real time PCR respectively. The release of ALP in 2 groups was compared. Cell viability is enhanced in Collagen-Hydroxyapatite scaffold after 7 days. SEM and DAPI staining show that cells are in good integration with structure of scaffold. Also, qRT-PCR of differentiated cells showed that COL-I, Osteocalcin (Bglap), bone sialoprotein (ibsp) and Runx-2 are expressed after 21days in 2D and 3D groups, in 3D group the expression of these markers were higher than 2D group. Under the effect of PMA the release of alkaline phosphatase increased incrementally in 3D group rather than 2D group during the 21 days of exposing the induction media. Based on our findings, Collagen-Hydroxyapatite Scaffold with PMA has the potential role in osteogenic differentiation of EnSCs and can be used to induce osteogenesis in bone defects.
Keywords of your Abstract :	purmorphamine-osteoblast like cells
Acceptance Letter :	http://gsia.tums.ac.ir/images/UserFiles/30943/Forms/306/acceptance_2.pdf
The presentation :	Oral
The Cover of Abstract book :	http://gsia.tums.ac.ir/images/UserFiles/30943/Forms/306/hamburg_2.pdf
Published abstract in the abstract book with the related code :	http://gsia.tums.ac.ir/images/UserFiles/30943/Forms/306/Abstract_Iranische Referenten_3.pdf
Where has your abstract been indexed? :	none
If you choose other, please name :

The Congress Reporting Form

How many volunteers were present at the Congress? :	proximal y 400
Delegates from which countries presented in the congress? :	IRAN-Netherlands-Japan-German- china
Were the delegates of any other organizations present in the congress? :	No
If yes, please write the names of the organizations in the box :
What were the responses to your talking points? Were specific questions or concerns raised? :	Most of the participants in the Congress of presentations were satisfied that take about new topics tissue engineering and repair of bone in the jaw . For example the effect of purmorphamine on differentiation of endometrial stem cells into osteoblast like cells on collagen-hydroxyapatite scaffolds
If you met staff members, please list their full names & positions. :	no
Please inform us if there are any follow up actions we need to talk with the members of the congress :	There are not any follow up actions you need to talk with the members of the congress.... Most of the participants in the Congress of presentations were satisfied that take about new topics tissue engineering and repair of bone in the jaw .
Your experiences about the travel processes(Providing ticket, accommodation,...) :	good
Please give a briefing of your own observations and outcomes of the congress: :	I enjoyed to see the novel research about craniomaxillo facial surgery and compared with our research. We must design the course about: trauma surgical methods tissue engineering stem cell ortho surgery and another case Most of the participants in the Congress of presentations were satisfied that take about new topics tissue engineering and stem cell and repair of bone and cartilage and mussels in the craniomaxillo facial surgery .

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