Code : 9345-352497      Created Date : Saturday, March 4, 2017   Visit : 1333

The report of 2nd Duden Conference/ 1st ISHAM-ECMM Aspergillus Resistance Surveillance working group meeting by Dr. Sadegh Khodaveisy

The report of 2nd Duden Conference/ 1st ISHAM-ECMM Aspergillus Resistance Surveillance working group meeting by Dr. Sadegh Khodaveisy
Application Code :
306-0117-0023
 
Created Date : Wednesday, February 8, 2017-21:26 21:26:26Update Date : Thursday, February 9, 2017-00:42 00:42:47
IP Address : 77.77.66.10Submit Date : Sunday, February 12, 2017-14:13 14:13:11Email :sadegh_7392008@yahoo.com
Personal Information
Name : Sadegh
Surname : Khodavaisy
School/Research center : School of Public Health
If you choose other, please name your Research center :  
Position : Assistant professor
Tel : +21-88-008588
Information of Congress
Title of the Congress : 2nd Duden Conference/ 1st ISHAM-ECMM Aspergillus Resistance Surveillance working group meeting
Title of your Abstract : Genotyping of clinical and environmental Aspergillus flavus isolates using AflaSTR assay and antifungal susceptibility patterns
Destination Country : Netherlands
From : Friday, January 20, 2017
To : Saturday, January 21, 2017
Abstract(Please copy/paste the abstract send to the congress) : Genotyping of clinical and environmental Aspergillus flavus isolates using AflaSTR assay and antifungal susceptibility patterns

Introduction: Aspergillus flavus is the second leading cause of fungal sinusitis, cutaneous infections, and endophthalmitis in tropical countries. Despite an increasing number of infections of A. flavus in Iran, the antifungal susceptibility patterns and molecular epidemiology of clinical and environmental strains has not been well studied. In the current study, we evaluated the in vitro antifungal susceptibilities of five antifungal agents and applying microsatellite typing in order to investigate the epidemiology of clinical and environmental A. flavus isolates from Iran.
Materials and methods: A. flavus strains were preliminarily identified to the species level based on microscopic and macroscopic characteristics; subsequently, their identity was confirmed by DNA sequencing of the partial β-tubulin gene. Antifungal susceptibilities of AMB, ITC, VRC, POS, and CAS against A. flavus isolates were determined in accordance with the CLSI M38-A2 document. The A. flavus isolates were genotyped by using microsatellite typing (AflaSTR assay). The genetic relatedness between the A. flavus isolates was investigated by comparing the profiles with BioNumerics v6.6 software. The discriminatory power of the microsatellite markers was calculated using Simpson’s index of diversity (D) as described previously.
Results: One hundred and ninety nine clinical (n = 171) and environmental (n= 28) A. flavus isolates were evaluated for their antifungal susceptibilities paterns. Caspofungin (MIC90=0.063 µg/mL), followed by posaconazole (MIC90=0.25 µg/mL), exhibited the lowest minimum inhibitory concentrations (MIC). All isolates had caspofungin MEC90 lower than the epidemiologic cutoff values, and 3.5% of the isolates had amphotericin B MIC higher than the epidemiologic cutoff values. Microsatellite typing of A. flavus isolates demonstrated 118 different genotypes. A possible outbreak at a pulmonary ward was discovered. The discriminatory power for the individual markers ranged from 0.4812 to 0.9457. 
Conclusion: Our results suggest that Iranian A. flavus isolates are the most susceptible to posaconazole and caspofungin, followed by voriconazole, itraconazole, and amphotericin B. Microsatellite typing provided excellent discriminatory power in studying the molecular epidemiology of clinical and environmental A. flavus isolates in Iran. 
Keywords of your Abstract : Antifungal susceptibility, Aspergillus flavus, Microsatellite strain typing, AflaSTR assay
 
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/23925/Forms/306/LOI_S_Khodavaisy_1_1.pdf
The presentation : Oral
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/23925/Forms/306/Abstract_book_cover.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/23925/Forms/306/Abstract_2.pdf
Where has your abstract been indexed? : none
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : About 90 volunteers.
Delegates from which countries presented in the congress? : Almost all countries such as Asia, The Netherlands, United States, Uk, Belgium, France.
Were the delegates of any other organizations present in the congress? : Yes
If yes, please write the names of the organizations in the box : Other Universities
What were the responses to your talking points? Were specific questions or concerns raised? : Several questions about the diagnostic and treatment options of Aspergillosis and antifungal resistance were asked. The questions were about the prevalence of Aspergillosis in different high risk patient. some question was about prevalence environmental and clinical route of azole resistance in our country. I answered them generally and explained.Also,the responses to my talking points were so interesting and unbelievable.
If you met staff members, please list their full names & positions. : I talked to many of experts in medical mycology and antifungal resistance from all of other countries and Iran.
Prof. Jacques Meis, The Netherlands
Dr. Anuradha chawdery, India
Dr. Hamid Badali, Iran
Dr. Katrien Lagrou, Belgium
Please inform us if there are any follow up actions we need to talk with the members of the congress : There is no specific action that could be follow up. However I personally talked with many researcher and negotiation with them for any possible collaboration. The main potential for collaboration was Prof. Jacques Meis from CWZ hospital in Nijmegen that works in our filed. 
We have valuable researches in our country. It will be useful to hold these meetings in our country and invite expertise from other countries.
Your experiences about the travel processes(Providing ticket, accommodation,...) : Everything went well, but It was so expensive. Also, paying registration fee and hotel booking was difficult as well because we do not have visa card
Please give a briefing of your own observations and outcomes of the congress: : This congress was a unique phenomenon happening for Antifungal resistance in Aspergillus spp. around the world to share their valuable experiences in this important field. The latest achievements in diagnosis of Aspergillosis and identification of resistance Aspergillus were discussed thoroughly during the specific congress. The Antifungal resistance, an entity that clinical should pay critical attention to it was discussed. It is crucial to remember that it is a significant problem throughout the world not only our country. I hope that there would be more chance of attending such international congresses to be able to share their valuable experience with experts around the world.

 

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