Code : 9345-352561      Created Date : Tuesday, March 14, 2017   Visit : 1394

The report of Biointerfaces International 2016, University of Zurich, August 23-25, 2016 by Dr. Mohammad Ali Faramarzi

The report of Biointerfaces International 2016, University of Zurich, August 23-25, 2016 by Dr. Mohammad Ali Faramarzi
Application Code :
306-0117-0033
 
Created Date : Wednesday, March 1, 2017-14:55 14:55:15Update Date : Wednesday, March 1, 2017-17:01 17:01:58
IP Address : 188.210.178.54Submit Date : Wednesday, March 1, 2017-17:02 17:02:45Email : faramarz@tums.ac.ir
Personal Information
Name : Mohammad Ali
Surname : Faramarzi
School/Research center : School of Pharmacy
If you choose other, please name your Research center :  
Position : Professor
Tel : +98-21-66954712
Information of Congress
Title of the Congress : Biointerfaces International 2016, University of Zurich, August 23-25, 2016
Title of your Abstract : Designing Targeted PLGA Nanoparticles with a Peptide Antagonist of VLA-4 as a Smart Drug Delivery System for Dysfunctional Endothelial Cells
Destination Country : Swaziland
From : Tuesday, August 23, 2016
To : Thursday, August 25, 2016
Abstract(Please copy/paste the abstract send to the congress) : INTRODUCTION: Dysfunctional endothelium is the initial and critical step of atherosclerosis. Rapid regeneration of damaged endothelial cells is expected to have a very important role in preventing of the disease. Discovery of smart drug delivery systems, directed against endothelium is essential due to its low access to imaging and pharmacological agents in the blood stream.
METHODS: Fluorescein isothiocyanate-loaded poly (DL-lactic-co-glycolic acid, PLGA) nanoparticles (NPs) were fabricated using double-nozzle electrospraying (at flow rate, DTAB salt concentration and polymer concentration values of 0.08 ml/h, 0.8 mM, and 0.7 w/v%, respectively). NPs were targeted directed against VCAM-1 with a peptide antagonist of VLA-4 (mZD7349 with sequence (cyclo(MePhe-Leu-Asp-Val-D-Arg-D-Lys)). Size, polydispersity index (PDI), zeta potential, and encapsulation efficiency (EE) of NPs and mZD7349-NPs were determined by Zetasizer. Rate of binding and internalization of mZD7349-NPs and NPs to activated human umbilical vein endothelial cells (HUVECs) by TNF-α (10 ng/ml, for 6h) were compared using fluorometry at 4ºC and 37ºC for incubation times of 30, 60, and 120 min.
RESULTS: Characteristics of NPs and mZD7349-NPs are shown in Table 1. The rate and the extent of binding of mZD7349-NPs were greater than NPs in all the three incubation times (Figure 1). Internalization of NPs and mZD7349-NPs decreased at 4ºC than to 37ºC.

Table 1. Characteristics of NPs (n=3, mean± SD).
Type Size (nm) PDI Zeta potential (mV) EE (%)
NPs 225± 14 0.47±
0.06 -11.7± 0.8 86.3±
6.5
mZD7349-NPs 229± 12 0.46±
0.07 -11.6± 1.1 69.6±
7.6

Figure 1. Rate of binding and internalization of mZD7349-NPs and NPs to activated HUVECs at 4ºC and 37ºC for times 30, 60, 120 min. mZD7349-NPs interacted faster and more with HUVECs compared with NPs at all time-points. Data are presented as mean ± S.D. (n=3), ٭,٭٭ indicate p ˂ 0.05, p ˂0.01, respectively.

DISCUSSION & CONCLUSIONS: Encapsulation efficiency in electrospraying is high and in this technic separation procedure of particles from the solvent is not required. Take up of ligand conjugated- NPs occurs by receptor-mediated endocytosis that occurs faster than the unconjugated NPs endocytosis. Internalization reduction of NPs and mZD7349-NPs at low temperature suggested energy dependent endocytosis of NPs. To conclude, mZD7394- NPs directed against VCAM-1 is suggested as a suitable carrier for atherosclerotic lesions upregulating VCAM-1.
REFERENCES: 1 K. Rani, S.A Paliwa (1991) Sch J App Med Sci 2:328-31. 2 V. Lassalle, M.L. Ferreira (2007) Macromol. Biosci 7:767-83. 3L.M. Bareford, P.W. Swaan (2007) Adv Drug Deliv Rev 59:748–58. 4 C. Chittasupho, P. Manikwar, J.P. Krise, et al. (2011) Mol Pharm 7:146.
ACKNOWLEDGEMENT: This research was supported by the grant No. 92-03-30-23890 from Tehran University of Medical Sciences.
Keywords of your Abstract : PLGA Nanoparticles, Peptide Antagonist of VLA-4, Smart Drug Delivery System, Dysfunctional Endothelial Cells
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/36090/Forms/306/Acceptance_letter.pdf
The presentation : Poster
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/36090/Forms/306/The_Cover_of_Abstract_book.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/36090/Forms/306/Published_Abstract.pdf
Where has your abstract been indexed? : ISI
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The Congress Reporting Form
How many volunteers were present at the Congress? : 400 people
Delegates from which countries presented in the congress? : Switzerland, Netherland, Germany, Canada, Sweden, Iran, France, USA, UK, Belgium, Jupon, etc.
Were the delegates of any other organizations present in the congress? : No
If yes, please write the names of the organizations in the box :  
What were the responses to your talking points? Were specific questions or concerns raised? : I got positive feedbacks about my research. The comments were mostly constructive. My presentation was related to different disciplines so could attract different scientists from several fields. Researchers specially early-stage investigators who were interested in medical microbiology and immunology approached me and gave comments.
If you met staff members, please list their full names & positions. : Marcus Textor, Scientific chair
Please inform us if there are any follow up actions we need to talk with the members of the congress : -
Your experiences about the travel processes(Providing ticket, accommodation,...) : All the arrangements were great. In Switzerland everything is on time and all the scientific events were held based on the conference schedule.
Please give a briefing of your own observations and outcomes of the congress: : The scope of the conference covered a wide range of research fields and the participants were from different disciplines. Biological interfaces are where synthetic/natural materials and biological systems interact with each other. This is one of the most innovative and expanding areas of science and technology. One the high-technology techniques that was presented in the conference was "3-D tissue printing". The technique can be used in tissue engineering and regenerative medicine and drug development in pharmaceutical industry. 3D tissue models are used as biologically relevant systems for preclinical compound screening and disease research. They help to identify potential toxic liabilities in an early phase of the drug discovery process and enable the reduction of animal experiments. Scaffold-free spheroids are going to be a new standard for preclinical in vitro testing. Microtissues produced with patient derived cells reflect the tissue heterogeneity and are potential suitable tools for drug development towards personalized medicine. Scaffold-based tissue models could be used as tool to investigate possible strategies for regenerative medicine. I have taken the information with me to share with my colleagues and students. Another presentation that attracted my attention was portable diagnostics using a combination of simple tools such as paper based platforms and bacteriophage-based detection. Paper-based devices, which have been proven to be a versatile platform for culture of mammalian or bacteria cells and detection of bacteria in resource limited environment. Another interesting talk was given by Dr. David Grainger on translational barriers to antimicrobial medical technologies. Increasing placements of medical devices now available for clinical implant use, often in aging populations and increasingly in developing countries, are a significant health care issue due to enhanced infection incidence intrinsically related to implanted medical devices. These devices are also implanted against a background of increasing bacterial antibiotic-resistance. Improvements in the prevention, diagnosis, and treatment of these device-associated infections will remain priority targets both for clinicians and the translational research community charged with addressing these challenges with innovation. Diverse approaches have been historically used to counter device-related infection, including antibiotic lavages, locally tethered or released antimicrobials, device coatings, local electric fields and current applications, and newer approaches targeting bacterial adhesion mechanisms, communication pathways, and virulence factors. Combination medical devices provide new innovative opportunities using local antibiotic formulations released from established classes of implants
This conference had a special format: it aimed to build bridges between the relevant research fields, between basic medical science and engineering, between scientific discovery, innovation and technology, and between senior scientists and young researchers. Both internationally highly renowned and young scientists from academia, industry and clinics presented their latest, often unpublished scientific findings and technological breakthroughs. All speakers began their talks with a general introduction that does not require specialized knowledge to follow. The focus of this conference was education across the many fields of relevance to biological surfaces and was particularly targeted at young researchers.
A full day was dedicated to translating science to clinics and to markets. Success stories of start-ups, corporate ventures and early-stage business ideas were presented, as well as insides of how to get innovation to clinics. Sessions topics of the 3-day conference program include surfaces & interfaces and its 
applications in Engineering Smart Surfaces / Bioinspired Responsive Interfaces, Smart (Drug) Delivery Systems, New Antibacterial Interface and Detection Strategies, Novel Interfacial Concepts in Bioanalytics & Diagnostics, 3D Cell Culture/Engineered Tissue Models, Biotribology, Progress in Biointerface Characterization and Imaging Methodologies, Translating science to clinics and to markets. Amazingly all the scientific sessions started on time!

 

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