|
Abstract(Please copy/paste the abstract send to the congress) : |
Meysam Ganjibakhsh1,6, Pouyan Aminishakib2,4, Parvaneh Farzaneh1, Abbas Karimi3, Seyed Abolhassan Shahzadeh Fazeli1,5, Moones Rajabi2, Ahmad Nasimian1,7, Fereshteh Baghaei4*, Hedieh Rahmati1, Neda sadat Gohari1, Nazanin Mohebali1, Masoumeh Asadi1, Zahra Elyasi Gorji1, Mehrnaz Izadpanah1,8, Shiva Mohamadi Moghanjoghi1, Sepideh Ashouri1
1 Human and Animal cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran
2 Department of Oral and Maxillofacial Pathology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
3 Department of Oral and Maxillofacial Surgery, Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
4 Dental Research Center, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran
5 Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran
6 Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
7 Department of Clinical Biochemistry, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
8 School of advanced technologies in medicine, Tehran University of Medical Sciences, Tehran, Iran
Objectives: Oral Squamous Cell Carcinoma (OSCC) is the most frequent oral cancer throughout the world. It is known as the eighth most common cancers in men and the fifth in women. Cytogenetic and biochemical studies in recent decades necessitated providing an appropriate material for such researches. Cancer cell culture is a useful tool for investigations on biochemical, genetic, molecular and immunological characteristics of different cancers, including OSCC.
Here, we explain the establishment process of five Iranian OSCC cell lines in details.
Materials & Methods: Specimens were obtained from 5 OSCC patients and enzymatic, explant culture and MACS methods were used for isolation cells. After confirmation of the quality control tests, characterization and authentication of OSCC cells were determined by STR, Chromosome analysis, Species identification, monitoring the growth, morphology and the expression of CD326 and CD133 markers.
Results: We could establish five OSCC cell lines from Iranian population with different purity which would provide an appropriate material for studies on cancer researches in future.
Conclusions: OSCC cell lines will provide an extremely useful platform for studying carcinogenesis pathways of OSCC in Iranian population and may explain the ethnic differences and the anti-cancer drug response in upcoming studies. |