Code : 9345-353628      Created Date : Saturday, November 18, 2017   Visit : 1596

The report of IPIC2017 - International Primary Immunodeficiencies Congress by Dr. Reza Yazdani

The report of IPIC2017 - International Primary Immunodeficiencies Congress by Dr. Reza Yazdani
Application Code :
762-0217-0088
 
Created Date : Tuesday, October 17, 2017-09:46 09:46:41Update Date : Saturday, November 18, 2017-12:28 12:28:55
IP Address : 194.225.212.155Submit Date : Saturday, November 18, 2017-12:29 12:29:16Email :reza_yazdani86@yahoo.com
Personal Information
Name : Reza
Surname : Yazdani
School/Research center : Research Center for Immunodeficiencies (RCI)
If you choose other, please name your Research center :  
Position : Assistant professor
Tel : +98-21-61472117
Information of Congress
Title of the Congress : IPIC2017 - International Primary Immunodeficiencies Congress
Title of your Abstract : EVALUATION OF PI3K/AKT/FOXO PATHWAY IN PATIENTS WITH COMMON VARIABLE IMMUNODEFICIENCY
Destination Country : United Arab Emirates
From : Sunday, October 8, 2017
To : Tuesday, October 10, 2017
Abstract(Please copy/paste the abstract send to the congress) : Objective: Common variable immunodeficiency (CVID) is the most frequent symptomatic primary immunodeficiency, characterized by hypogammaglobulinemia. PI3K/AKT/FOXO pathway has important role in survival and differentiation of B-cells. Since defect in this pathway could be involved in defective survival and differentiation of B-cells, we evaluated this pathway on B-cells from CVID patients.

Design and Methods: B-cells from 10 patients and 10 healthy individuals were purified by negative selection and were stimulated by anti-IgM and anti-CD40 antibody for 24 hours. We evaluated protein and gene expression of PI3K, AKT and Foxo molecules in B-cells by flowcytometry and real-time PCR, respectively. Moreover, the level of phosphorilated AKT in B-cells has also been measured by flowcytometry. Furthermore, spontaneous and induced apoptosis of B cells have been evaluated. Four-color flow cytometric immunophenotyping determinations of B-cell subsets were also performed using FACSCalibur.

Results: We have not identified significant difference between protein and gene expression of PI3K, AKT and Foxo molecules in B-cells from patients than controls. However, we surprisingly found phosphorylated Akt (p-AKT) levels are significantly lower in B-cells from patients compared with controls. Moreover, our results demonstrate increased spontaneous and induced apoptosis of B cells in patients. Furthermore, our patients presented a significant reduction in B-cell subset numbers than normal cases. 

Conclusions: Our results suggest that impairment in phosphorilation of AKT leads to induction of apoptosis in B-cells from CVID patients. Thus, defective p-AKT and increased apoptosis leads to abnormality in B-cell subset numbers, as B cells could be unable to complete their maturation and differentiation. 
 
Keywords of your Abstract : Common Variable Immunodeficiency, Signaling, B-cells
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/41353/Forms/762/Acceptance.pdf
The presentation : Poster
The Cover of Abstract book :
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/41353/Forms/762/Abstract_in_book.pdf
Where has your abstract been indexed? : none
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : More than 400 participants
Delegates from which countries presented in the congress? : United Kingdom, USA, India, Italy, poland
Were the delegates of any other organizations present in the congress? : Yes
If yes, please write the names of the organizations in the box : IPOPI
What were the responses to your talking points? Were specific questions or concerns raised? : Yes, I discussed with other scientist about our results and they gave me valuable comments. Also, I spoke about future studies with famous researchers and I could make a contact with them for collaborating in next studies in future.
If you met staff members, please list their full names & positions. : 1. Prof.Klaus Warnatz, Group Leader, Center for Chronic Immunodeficiency, Faculty of Medicine - University of Freiburg, Germany
Scientific Committe in IPIC congressa
2. Dr Mirjam Van der burg, Group Leader, Laboratory Medical Immunology, Department of Immunology, Rotterdam Area, Netherlands

3. prof Bodo Grimbacher,Group Leader, Genetic basis of immunodeficiency, Center for Chronic Immunodeficiency
at Center for Translational Cell Research
Please inform us if there are any follow up actions we need to talk with the members of the congress :  
Your experiences about the travel processes(Providing ticket, accommodation,...) : There are many hotels in Dubai and someone has many options to select a hotel. Totally, accommodation was almost expensive in Dubai. However, the flight ticket was cheap. location of congress was very nice and I enjoyed from location and organization of congress.
Please give a briefing of your own observations and outcomes of the congress: : New findings in diagnosis and treatment of PID were described in this congress that were very useful and helpful in better diagnosis and management of PID patients. Also, I could made a contact with some famous researchers in this congress about my next studies in future.

 

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