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Code : 9822-354015 Created Date : Wednesday, February 14, 2018 Visit : 2083

The report of Microscopy Conference 2017 by Samira Elikaee

Application Code :

762-0217-0060

Created Date : Tuesday, September 19, 2017-08:16 08:16:00Update Date : Wednesday, February 14, 2018-08:57 08:57:25
IP Address : 194.225.57.35Submit Date : Wednesday, February 14, 2018-08:57 08:57:40Email :samira_ele@yahoo.com

Personal Information

Name :	Samira
Surname :	Elikaee
School/Research center :	School of Public Health
If you choose other, please name your Research center :
Position :	staff
Tel :	+21-224-05174

Information of Congress

Title of the Congress :	Microscopy Conference 2017
Title of your Abstract :	Evaluation of p27 gene knockout mutant of Leishmania major (MRHO/IR/75/ER) with Light and Electron Microscopy
Destination Country :	Switzerland
From :	Monday, August 21, 2017
To :	Friday, August 25, 2017
Abstract(Please copy/paste the abstract send to the congress) :	Leishmaniasis as a parasitic disease with manifestations ranging from cutaneous to fatal visceral infection is caused by several Leishmania species. These protozoan parasites in the gut of a sandfly vector or in culture media replicate as extracellular, flagellated promastigotes and inside the parasitophorous vacuole of vertebrate host macrophages as amastigotes. In Iran Zoonotic Cutaneous Leishmaniasis (ZCL) caused by Leishmania major (L. major). L. major p27 is an important gene encoding cytochrome c oxidase (COX) component and is a mitochondrial membrane protein. It expresses a 27 kDa protein that has potential role in ATP synthesis. This study aimed to evaluate morphology of L. major mutant (deficient in the p27 gene) with light and Scanning electron microscopy (SEM) imaging methods and its pathogenicity in BALB/c mice. Evaluation of L. major mutant promastigotes with invert light microscope (400X) showed that proliferation and differentiation is slower than virulent ones but in term of morphology with light microscope (1000X) and Gimsa staining, we didn’t see any difference in nucleus, kinetoplast, body and flagella between them. Results of Scanning electron microscopy also demonstrated no difference between body and flagellum although the parasites have a highly resolution in cell shape and form. Also in BALB/c mice the mutant didn’t cause any lesion and in 75% of them we didn’t see any amastigote in their smears (1000X). In conclusion, there is no difference between Knockout and Virulent L. major with light microscopic and SEM methods. It seems Transmission electron microscopy (TEM) is more useful in analysis of morphology and differentiation of produced p27 gene knockout mutant.
Keywords of your Abstract :	Leishmania major; electron microscopy; Light microscopy; knockout; p27gene
Acceptance Letter :	http://gsia.tums.ac.ir/images/UserFiles/40785/Forms/762/MC2017 - Acceptance Letter_Elikaee (1) (1) (1)_2.pdf
The presentation :	Poster
The Cover of Abstract book :	http://gsia.tums.ac.ir/images/UserFiles/40785/Forms/762/Cover1.pdf
Published abstract in the abstract book with the related code :	http://gsia.tums.ac.ir/images/UserFiles/40785/Forms/762/Abstract.pdf
Where has your abstract been indexed? :	ISI
If you choose other, please name :

The Congress Reporting Form

How many volunteers were present at the Congress? :	1000
Delegates from which countries presented in the congress? :	Switzerland- Germany- Austria- Japan- Sweden
Were the delegates of any other organizations present in the congress? :	Yes
If yes, please write the names of the organizations in the box :	German Society for Electron Microscopy
What were the responses to your talking points? Were specific questions or concerns raised? :	They asked me about the number of cases(patients) infected to the forms of cutaneous leishmaniasis and also the asked me for more explain about the way for preventation such cases through vaccination protocol. Also they wanted more explain about the results of knockout leishmania.
If you met staff members, please list their full names & positions. :	Marco Cantoni; Rolf Erni; Markus Durrenberger, Chair and Co chair
Please inform us if there are any follow up actions we need to talk with the members of the congress :	As Iran is an endemic area for Leishmaniasis so I think it will be a good opportunity for us to be the host for the special symposium in the Leishmaniasis background in future and inviting of these professors to have speech for us will help us to use their experience in the field and have common projects.
Your experiences about the travel processes(Providing ticket, accommodation,...) :	Providing ticket, accommodation was some difficult and expensive, all parts needed visa card and in the city I must did shopping and travelling just in cash.It is very perfect if there is a section in international affairs for helping the participants for ticket and paying the registration fee and reserving hotel.
Please give a briefing of your own observations and outcomes of the congress: :	This international conference organized every 4 years. This conference in Lausanne conducted under the auspices of the European Microscopy Society (EMS) containing three main topical areas addressing advances in instrumentation, life sciences and materials sciences. I think the conference is more powerful in material sciences. The location was an attractive place for scientific events, located at shore of lake Geneva

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