Intl. Congress form | International Congress Report | International Congress Report For Faculty | The report of Microscopy Conference 2017 by Dr. Homa Hajjaran

Home

Code : 9345-354035 Created Date : Monday, February 19, 2018 Visit : 1716

The report of Microscopy Conference 2017 by Dr. Homa Hajjaran

Application Code :

762-0217-0066

Created Date : Monday, October 9, 2017-14:53 14:53:56Update Date : Sunday, January 21, 2018-16:02 16:02:42
IP Address : 194.225.57.36Submit Date : Wednesday, February 14, 2018-12:42 12:42:51Email : hajaranh@tums.ac.ir

Personal Information

Name :	Homa
Surname :	Hajjaran
School/Research center :	School of Public Health
If you choose other, please name your Research center :
Position :	Professor
Tel :	+98-21-88951392

Information of Congress

Title of the Congress :	Microscopy Conference 2017
Title of your Abstract :	Identification of Cytoskeleton and cellular traffic and membrane dynamics of Iranian Leishmania infantum by LC- Mass spectrometry
Destination Country :	Switzerland
From :	Monday, August 21, 2017
To :	Friday, August 25, 2017
Abstract(Please copy/paste the abstract send to the congress) :	Introduction: Leishmania parasites cause a broad spectrum of clinical disease. The outcome of infection is influenced of both host immune situation and the parasite species. The mechanisms of species differences and virulence are under the influence of gene expression regulation at post transcriptional stages. Proteomic studies by using the 2 dimentional electrophoresis (2-DE) and mass-spectrometry increase our understanding of the mechanism of the physiological or pathological process. Objectives: Here in, we report the dynamic roles of Cytoskeleton and cellular traffic in L. infantum through the 2-DE and liquid mass spectrometry (LC-MS). Materials and Methods: As a preliminary study, we compared the proteome mapping of the Iranian isolate of Leishmania infantum through the (2-DE), and identified the prominent proteins by Liquid Chromatography (LC) mass spectrometry. Results: We reproducibly detected about 700 protein spots in L. infantum by using the Melanie software. Of the 135 proteins that were detected on the analytical gels, 61 proteins were detected reliably on CBB-stained preparative gels which were analyzed using LC/MS/MS after excitation from CBB stained gels. These proteins according to their functions and biological processes were classified in twelve categories: carbohydrate metabolism process, protein synthesis and assembly, cell signaling and vesicular trafficking, intracellular survival/proteolysis, antioxidant defense, stress related proteins/protein folding, cell motility and cytoskeleton, nucleoside, nucleotide and nucleic acid metabolism. Conclusion: One of the cell signaling proteins was calmodulin-like protein. Calmodulin is a kind of calcium binding protein which expresses in all eukaryotic cells including members of the genus Leishmania; it participates in calcium signaling pathways that regulate multiple critical processes such as growth and proliferation. Moreover, it plays a vital role in virulence of Leishmania during invasion of macrophage. On the other hand vacuolar sorting-like protein as a vesicular trafficking protein involved in sorting and delivering of vacuolar proteins to each intracellular compartment. Also this protein as a member of endosome sorting and authophagy pathways is essential for differentiation and virulence of Leishmania.
Keywords of your Abstract :	Leishmania infantum, Proteomics, Cytoskeleton and cellular traffic, @-DE, LC- Mass spectrometry.
Acceptance Letter :	http://gsia.tums.ac.ir/images/UserFiles/21808/Forms/762/acceptance_swiss.pdf
The presentation :	Poster
The Cover of Abstract book :	http://gsia.tums.ac.ir/images/UserFiles/21808/Forms/762/cover abstract book_1.pdf
Published abstract in the abstract book with the related code :	http://gsia.tums.ac.ir/images/UserFiles/21808/Forms/762/abstract.pdf
Where has your abstract been indexed? :	ISI
If you choose other, please name :

The Congress Reporting Form

How many volunteers were present at the Congress? :	About 700
Delegates from which countries presented in the congress? :	Swiss- Germany- Austria - Italy- and other European countries
Were the delegates of any other organizations present in the congress? :	Yes
If yes, please write the names of the organizations in the box :	Swiss Society for Electron microscopy, ASEM Austria Society for Electron Microscopy- DGE German Society for Electron Microscopy
What were the responses to your talking points? Were specific questions or concerns raised? :	They asked me about the number of cases(patients) infected to the resistant forms of cutaneous leishmaniasis to Meglumine antimoniate and also the asked me for more explain about the way for diagnosing such cases through the best biomarker for follow up the treatment protocol. Also they wanted more explain about the results of Mass spectrometry.
If you met staff members, please list their full names & positions. :	Conference Chairs : Marco Cantoni from Swiss, Rolf Erni from Swiss and Markus Durrenberger from Basel Swiss
Please inform us if there are any follow up actions we need to talk with the members of the congress :	As Iran is an endemic area for Leishmaniasis so I think it will be a good opportunity for us to be the host for the special symposium in the Leishmaniasis background in future and inviting of these professors to have speech for us will help us to use their experience in the field and have common projects.
Your experiences about the travel processes(Providing ticket, accommodation,...) :	I,m pleased to informed you I arrived first in Germany ( Berlin ) for visiting my son and then I went to Swiss for participation in MC conference. All of the trip and accommodation and designed programmed of the congress was perfect. Already my son reserved hotel and internal tickets. It is very perfect if there is a section in international affairs for helping the participants for ticket and paying the registration fee and reserving hotel.
Please give a briefing of your own observations and outcomes of the congress: :	All of the lectures and posters of the congress were very professional and were presented perfect. In beside the congress there were many perfect Microscopy company with the best technology. As Iran is an endemic area for some of parasitic diseases like leishmaniasis and Malaria so we can cooperate with each other to reach the best way to study the basic science for parasites. More interesting to me was the opportunity to have a practical experience with the recent advanced technologies. So I try to make such proposal with collaboration of such researcher in European countries specially Germany for such issues.

Home Printer Friendly Tell a Friend Comment

Your Comments :