Tehran University of Medical Sciences
Office of Vice-Chancellor for Global Strategies & International Affairs
International Human Capacity Development (IHCD)
Code : 9345-352845      Publish Date : Sunday, May 28, 2017 Visit : 1488

Intl. Congress form | International Congress Report | International Congress Report For Faculty | "Malaria: From Innovation to Eradication" by Dr. Afsaneh Motevalli Haghi

"Malaria: From Innovation to Eradication" by Dr. Afsaneh Motevalli Haghi
"Malaria: From Innovation to Eradication" by Dr. Afsaneh Motevalli Haghi
 
Application Code :
306-0117-0044
 
Created Date : Tuesday, March 7, 2017-09:05 09:05:05Update Date : Wednesday, May 10, 2017-15:20 15:20:40
IP Address : 194.225.57.35Submit Date : Wednesday, May 10, 2017-15:20 15:20:57Email : amh.mot@gmail.com
Personal Information
Name : Afsaneh
Surname : Motevalli Haghi
School/Research center : School of Public Health
If you choose other, please name your Research center :  
Position : Assistant professor
Tel : +98-21-42933256
Information of Congress
Title of the Congress : "Malaria: From Innovation to Eradication"
Title of your Abstract : Allelic variations of Plasmodium vivax Apical Membrane Antigen -1 (Pv AMA-1) in malarious areas of southeastern Iran using PCR -RFLP technique.
Destination Country : Uganda
From : Thursday, February 18, 2016
To : Wednesday, February 24, 2016
Abstract(Please copy/paste the abstract send to the congress) : Allelic variations of Plasmodium vivax Apical Membrane Antigen -1 (Pv AMA-1) in malarious areas of southeastern Iran using PCR -RFLP technique.

Afsaneh MOTEVALLI HAGHI *1, Mehdi NATEGHPOUR 1, Gholamhossein EDRISSIAN1, Sepide MORADI1
1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
*corresponding author: Tel: +989121392574, E-mail: a-motevalli@tums.ac.ir
Abstract:
Background: Although Plasmodium vivax is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. Studies on the genetic variations in P.vivax can guide relevant authors to predict the future situation of different strains of the Plasmodium in view of treatment, control, vaccine production and drug resistance. In this study we tried to determine some genetic variations in PvAMA-1 antigen among the samples were collected form southeastern Iran. 
Methods: About two ml blood samples were collected into EDTA pre-dosed tubes from 36 P.vivax–infected patients individually. Previous to the above blood collection process a Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen. 
Results: A 1300bp gene of the antigen was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite.
Conclusion: This study increased our data about prevalence and variation of PvAMA-1 alleles amongst P.vivax isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants.
Key words: PvAMA-1, PCR-RFLP, Allelic variations, Iran
 
Keywords of your Abstract : Key words: PvAMA-1, PCR-RFLP, Allelic variations, Iran
Acceptance Letter : http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/acceptance2.pdf
The presentation : Poster
The Cover of Abstract book : http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/cover of book_1.pdf
Published abstract in the abstract book with the related code : http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/Abstract.pdf
Where has your abstract been indexed? : ISI
If you choose other, please name :  
The Congress Reporting Form
How many volunteers were present at the Congress? : 300 volunteers
Delegates from which countries presented in the congress? : Swiss, USA, Nigeria, Kenya, Sudan, Serilanka, India, Southafrica, England
Were the delegates of any other organizations present in the congress? : No
If yes, please write the names of the organizations in the box : -
What were the responses to your talking points? Were specific questions or concerns raised? : There was an important section to discuss about posters. Molecular approaches were interesting for participants.One major question about our study was : What is the next step for this activity?
If you met staff members, please list their full names & positions. : Sara Volkman: Professor in Harvard.
Marcel Tanner: Swiss tropical and public health institute.
Please inform us if there are any follow up actions we need to talk with the members of the congress : Thank you for your kindly cooperation. In fact we need international coordinate to solve problem in our country.
Your experiences about the travel processes(Providing ticket, accommodation,...) : The best issue was to gain new research in my specific working field on malaria.
Please give a briefing of your own observations and outcomes of the congress: : There were a lot of new study design and methods to apply for our students to design their projects.