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Title of the Congress : |
"Malaria: From Innovation to Eradication" |
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Title of your Abstract : |
Allelic variations of Plasmodium vivax Apical Membrane Antigen -1 (Pv AMA-1) in malarious areas of southeastern Iran using PCR -RFLP technique. |
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Destination Country : |
Uganda |
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From : |
Thursday, February 18, 2016 |
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To : |
Wednesday, February 24, 2016 |
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Abstract(Please copy/paste the abstract send to the congress) : |
Allelic variations of Plasmodium vivax Apical Membrane Antigen -1 (Pv AMA-1) in malarious areas of southeastern Iran using PCR -RFLP technique.
Afsaneh MOTEVALLI HAGHI *1, Mehdi NATEGHPOUR 1, Gholamhossein EDRISSIAN1, Sepide MORADI1
1. Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
*corresponding author: Tel: +989121392574, E-mail: a-motevalli@tums.ac.ir
Abstract:
Background: Although Plasmodium vivax is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. Studies on the genetic variations in P.vivax can guide relevant authors to predict the future situation of different strains of the Plasmodium in view of treatment, control, vaccine production and drug resistance. In this study we tried to determine some genetic variations in PvAMA-1 antigen among the samples were collected form southeastern Iran.
Methods: About two ml blood samples were collected into EDTA pre-dosed tubes from 36 P.vivax–infected patients individually. Previous to the above blood collection process a Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen.
Results: A 1300bp gene of the antigen was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite.
Conclusion: This study increased our data about prevalence and variation of PvAMA-1 alleles amongst P.vivax isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants.
Key words: PvAMA-1, PCR-RFLP, Allelic variations, Iran
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Keywords of your Abstract : |
Key words: PvAMA-1, PCR-RFLP, Allelic variations, Iran |
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Acceptance Letter : |
http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/acceptance2.pdf |
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The presentation : |
Poster |
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The Cover of Abstract book : |
http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/cover of book_1.pdf |
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Published abstract in the abstract book with the related code : |
http://gsia.tums.ac.ir/images/UserFiles/21890/Forms/306/Abstract.pdf |
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Where has your abstract been indexed? : |
ISI |
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If you choose other, please name : |
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