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Code : 9822-331825 Created Date : Tuesday, November 5, 2013 Update Date : Tuesday, November 5, 2013 Visit : 5381

14th International Conference on Pseudomonas

The Report of 14th International Conference on Pseudomonas by Leila azimi

Application Code :

306-0213-0023

Created Date : Sunday, September 29, 2013Update Date : Sunday, September 29, 2013IP Address :188.245.248.174
Submit Date : Sunday, September 29, 2013Email : leilaazimi1982@gmail.com

Personal Information

Name :	Leila
Surname :	Azimi
School/Research center :	Antimicrobial Resistance Research Center, Tehran niversity of Medical Sciences
Possition :	student
Tel :	+98-21-64352397
E-mail :	leilaazimi1982@gmail.com

Information of Congress

Title of the Congress :	14th International Conference on Pseudomonas
Title of your Abstract :	Comparison between phenotypic and genotypic methods for detection of KPC-producer Pseudomonas aeruginosa
Venue :	LAUSANNE, SWITZERLAND
From :	Saturday, September 07, 2013
To :	Wednesday, September 11, 2013
Abstract(Please copy/paste the abstract send to the congress) :	ID: 15 Leila Azimi Comparison between phenotypic and genotypic methods for detection of KPC-producer Pseudomonas aeruginosa Leila Azimi1, AbdolaizRastegar Lari1, Babak Asghari1, Mina Bustanshenas1, Faranak Alinejad2 Antimicrobial Resistance Research Center, Tehran University of Medical Sciences, Tehran, Iran1, Motahhari Burn Research Center, Tehran University of Medical Sciences, Tehran, ISLAMIC REPUBLIC OF IRAN2 Background: Pseudomonas aeruginosa is an important opportunistic pathogen causing nosocomial infections in burned patients and make an important concern in care systems. Carbapenems are used for treatment of infections caused by multi drug resistance (MDR) P. aeruginosa. Production of Klebsiella pneumonia carbapenemase (KPC) is one of the mechanisms of resistance to carbapenems in MDR P. aeruginosa. The aim of this study was comparison of phenotypical methods, Modified Hodge Test (MHT) and use of boronic acid (BA) as an inhibitor and molecular test (PCR) for detection of KPC - producer P. aeruginosa. Materials and methods: In the present study 174 carbapenem resistant P. aeruginosa were isolated from hospitalized burned patients and identified by biochemical tests. According to CLSI, MHT was conducted for all isolates and synergism test between meropenem and BA was tested in strains with clover life shape in MHT. Synergism effect of meropenem and cloxacillin was calculated for removal false positive responds in BA positive test. Finally, PCR assay was performed with 5 different specific primers for molecular identification of kpc gene. Results: Seventy strains have MHT positive. Ten out of 70 have synergism effect between meropenem and BA. BA has inhibition activity alone only in 3 isolates and 7 strains have disparity in inhibition zone by cloxacillin too. Two of 70 MHT test positive strains showed the specific amplification fragment for kpc gene which is only one of them has synergism effect with BA. Conclusion: MHT can use for primary screening of carbapenemases producer P. aeruginosa for excellent sensitivity but has low specificity for detection of KPC. Also, only 3 MHT positive isolates have positive synergism test with BA and just one strain among them have specific band after PCR. In conclusion PCR method is nevertheless a golden standard for this aim.
Keywords of your Abstract :	Pseudomonas, KPC, phenotypic methods, genotypic methods
Acceptance Letter :	http://gsia.tums.ac.ir/images/UserFiles/11575/Forms/306/Lettre_Ambassade_AZIMI_13_05_12_1_.pdf
The presentation :	Poster
The Cover of Abstract book :
Published abstract in the abstract book with the related code :
Where has your abstract been indexed? :	none
If you choose other, please name :

The Congress Reporting Form

How many volunteers were present at the Congress? :	420
Delegates from which countries presented in the congress? :	Lausanne University Professors, students and researchers
Were the delegates of any other organizations present in the congress? :	Yes
If yes, please write the names of the organizations in the box :	FEMS
What were the responses to your talking points? Were specific questions or concerns raised? :	importance role of Pseudomonas in infections and spread of MDR species of them in nosocomoial infections. Some especial characterization of this bacteria such as it's pigments and reasons of exist of different color of them.
If you met staff members, please list their full names & positions. :	Pr. Patrick Plesiate
Please inform us if there are any follow up actions we need to talk with the members of the congress :	you can download abstract book in your site for other colleges. on the other hand, you can identify some famous researchers in the congress and find their contact mail for invite them to our congress in Iran and use from their update and useful informations. the E. mail of staff of congress is in the sit of congress and you can contact them for get more information about especial person or some questions about international congress. congress informations: 14TH INTERNATIONAL CONFERENCE ON PSEUDOMONAS LAUSANNE, SWITZERLAND, 07-11 SEPT 2013 Martine Moreillon & Irene Müller Secretariat conference website www.unil.ch/pseudomonas2013
Your experiences about the travel processes(Providing ticket, accommodation,...) :	I have reserved my hotel and train ticket by internet and it make easy for me for accommodation and prepare ticket.
Please give a briefing of your own observations and outcomes of the congress: :	I could visit Pr. Patrick Plesiate (Chief of Ph.D school in Besancon, France). Inform new documents about Pseudomonas word wide. Get good idea about some parts of my thesis. we can share our data and documents in that congress. we can visit another Iranian researcher in another countries and share contact information of ours for next contribution. I can get more information about Pseudomonas infections around the word and compare their results with ours. some aspects of Pseudomonas were discussed in this congress that we did not attention to them. congress was formation in one hall and has 3 halls for the posters. when we enter to registration hall we faced to registration desk and then one desk for tea, coffee and cake. They had 2 sections in the morning, then break point and then another section before lunch. 2 sections were presented in afternoon and then break point and after that 2 another sections were presented.

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