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Title of the Congress : |
The 2ndINTERNATIONAL CONFERENCE ON HEART & BRAIN |
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Title of your Abstract : |
INFLUENCE OF VITAMIN A SUPPLEMENTATION ON CYTOKINES GENE EXPRESSION THAT SECRETED BY TCD4+ LYMPHOCYTES IN ATHEROSCLEROTIC PATIENTS |
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country : |
france |
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From : |
Thursday, February 27, 2014 |
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To : |
Saturday, March 01, 2014 |
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Abstract(Please copy/paste the abstract send to the congress) : |
INFLUENCE OF VITAMIN A SUPPLEMENTATION ON CYTOKINES GENE EXPRESSION THAT SECRETED BY TCD4+ LYMPHOCYTES IN ATHEROSCLEROTIC PATIENTS
S.A. Keshavarz, A.A. Saboor-Yaraghi, A. Mottaghi, K. Mirzaei
School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran.
Aim
Atherosclerosis is an inflammatory arterial wall disease and T lymphocytes have important role in the pathogenesis and progression of this disease. The aim of this study is determination of vitamin A supplementation effects on gene expression of cytokines secreted by TCD4+ lymphocytes in atherosclerotic patients.
Methods and Materials: Thirty one atherosclerotic patients and 12 healthy controls participated in this study. Vitamin A supplement was given as retinyl palmitate; 25000 IU per day. After the intervention period the gene expression pattern of relevant cytokines of CD4+ T cells including Th1, Th2, Th17 and Treg were determined by Real-Time PCR from peripheral blood mononuclear cells.
Results: Vitamin A increased proliferation of cells that stimulated with ox-LDL in all groups. Results of this study show that IFN-y and T- bet gene expression in fresh cells in vitamin A-treated patients was decreased (0.85 fold, P=0.068 and 0.39 fold, P=0.0001; respectively). The IL-4 gene expression were increased 12.7 fold (P=0.0001). 2.9 fold (P=0.323) and 3.5 fold (P=0.009) in vitamin A-treated patients, patients who received placebo and healthy controls respectively. IL-17 gene expression in fresh cells of vitamin A-treated patients were diminished (0.63 fold, P=0.003).
Gene expression of RORc in PHA-activated cells in vitamin A-treated patients and healthy controls was decreased (0.87 fold. P=0.015 and 0.72 fold, P=0.022; respectively) but in patients who received placebo was elevated (1.17 fold, P=0.019) in this gene expression was seen. Foxp3 gene expression in fresh cells was increased after intervention in all groups (Vitamin A-treated patients 10.1 fold (P=0.0001), patients who received placebo 1.9 fold (P=0.254) and healthy controls 5.6 fold (P=0.0001).
Conclusion: Increase in gene expression of Th2 cells was seen in all group expect GATA3 gene. According to the results of how the effect of vitamin A on gene expression in atherosclerotic patients, perhaps we thought the positive role of vitamin A supplementation in these patients. Results of this study could pave the way for a more detailed review on effect of vitamin A in patients with immune related diseases. |
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Keywords of your Abstract : |
Atherosclerosis, vitamin A supplementation, TCD4+ lymphocytes |
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Acceptance Letter : |
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The presentation : |
eposter |
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The Cover of Abstract book : |
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Published abstract in the abstract book with the related code : |
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Where has your abstract been indexed? : |
none |
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