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Code : 9345-350510 Created Date : Sunday, November 1, 2015 Update Date : Sunday, November 1, 2015 Visit : 1798

9th Europian congress of tropical medicine and international health

The report of 9th European congress of tropical medicine and international health by Dr. Saeedeh Shojaee

Application Code :

306-0215-0106

Created Date : Monday, September 21, 2015 09:53:47Update Date : Saturday, October 3, 2015 12:17:17
IP Address : 194.225.57.25Submit Date : Saturday, October 3, 2015 12:17:26Email : shojaee1980@yahoo.com

Personal Information

Name :	saeedeh
Surname :	shojaee
School/Research center :	School of Public Health
If you choose other, please name your Research center :
Position :	Associate professor
Tel :	+98-21-88951392

Information of Congress

Title of the Congress :	9th Europian congress of tropical medicine and international health
Title of your Abstract :	Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for detection of toxoplasmosis by ELISA test
Country :	Switzerland
From :	Sunday, September 6, 2015
To :	Thursday, September 10, 2015
Abstract(Please copy/paste the abstract send to the congress) :	Evaluation of Toxoplasma gondii soluble, whole and excretory/secretary antigens for detection of toxoplasmosis by ELISA test S. Shojaee *.S. Pishkari, H. Keshavarz, M. Salimi, M. Mohebali School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Corresponding author: shojaee1980@yahoo.com The present study was performed to compare the soluble, whole and excretory/secretary antigens of Toxoplasma gondii (RH strain) in diagnosis of toxoplasmosis by ELISA method. Tachyzoites of T.gondii, RH strain were injected in intra-peritoneal cavity of BALB/c mice, after 4 days tachyzoites were harvested by peritoneal washing of the mice. For soluble antigen, exudates were centrifuged and sediment sonicated and then centrifuged at 4ºC, 1hr, supernatant collected and density of protein determined by Bradford method. For whole antigen after collecting, washing and centrifuging of peritoneal fluid the tachyzoites sediment was counted. In excretory/secretary antigen 1.5 × tachyzoites were transferred in 1ml tube of saline and incubated under mild agitation and after centrifuging, supernatant was collected and protein density determined by Bradford method. 176 human serum samples were evaluated for T.gondii IgG antibody with prepared antigens, and finally serum samples were evaluated by commercial ELISA kit (Trinity,USA) which was considered as gold standard method. In this study sensitivity and specificity of prepared antigens compared with commercial kit in ELISA method. Sensitivity and specificity of soluble antigen was 91.4% and 74.5%, in whole antigen these parameters were 77.1% and 77.3% and in excretory/secretary antigen were 28.5% and 74.5% respectivly. Soluble antigen had high levels of sensitivity and specificity in ELISA method and the results were rather resemble to commercial kit (Trinity,USA). Key words: Toxoplasmosis, ELISA, Toxoplasma gondii, Soluble antigen, Whole antigen, Excretory/secretary antigen.
Keywords of your Abstract :	Toxoplasmosis, ELISA, Toxoplasma gondii, Soluble antigen, Whole antigen, Excretory/secretary antigen
Acceptance Letter :	http://gsia.tums.ac.ir/images/UserFiles/21831/Forms/306/On_Tuesday.pdf
The presentation :	Poster
The Cover of Abstract book :	http://gsia.tums.ac.ir/images/UserFiles/21831/Forms/306/You_have_free_access_to_this_content.pdf
Published abstract in the abstract book with the related code :	http://gsia.tums.ac.ir/images/UserFiles/21831/Forms/306/abs_book.pdf
Where has your abstract been indexed? :	ISI
If you choose other, please name :

The Congress Reporting Form

How many volunteers were present at the Congress? :	2000 delegates
Delegates from which countries presented in the congress? :	102 countries including Germany,India,France,Australia,South Korea
Were the delegates of any other organizations present in the congress? :	Yes
If yes, please write the names of the organizations in the box :	iranian pasteur institute
What were the responses to your talking points? Were specific questions or concerns raised? :	The responses to my talking points were very good. One researcher from Sweden asked me questions about the method that i had used in my research.
If you met staff members, please list their full names & positions. :	Imet some of experts in international health and medical parasitology
Please inform us if there are any follow up actions we need to talk with the members of the congress :	Because we have no type of credit cards for taking part in the congress, so we cannot register before primary deadline of the congress and we should pay more money ,so preparing some facilities such as payments of mission charges is necessary or some contacts with secretariat of the congress to explain the situation.
Your experiences about the travel processes(Providing ticket, accommodation,...) :	very difficult and expensive
Please give a briefing of your own observations and outcomes of the congress: :	The 9th European congress of tropical medicine and international health was held in Basel, Switzerland from 6 up to 10th September, 2015. This congress is held every other year in different countries of Europe. Observation of great number of different scientists and researchers from different countries from worldwide was so wonderful and attractive for me because it could be increased the enthusiasm of research and study. It was interesting for them that researchers from Iran were attended in this congress too.

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