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Abstract(Please copy/paste the abstract send to the congress) : |
Abstract
The effect of nitrazepam on depression and curiosity in behavioral tests
in mice: The role of potassium channels
Azam Bakhtiarian, PhD, Dept of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Seyyedeh Elaheh Mousavi, PhD, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Farahnaz Jazaeri, PhD, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Vahid Nikoui, PhD, Razi Institute for Drug Research, Iran University of Medical Sciences, Tehran, Iran
Evidence show that gamma-aminobutyric acid
(GABA) receptors are involved in
depression, so the aim of this study was to
investigate the effect of nitrazepam as
agonist of GABAA receptors on depression
and curiosity in male mice and the role of
potassium channel in antidepressant-like
response. For this purpose, we studied the
antidepressant-like properties of fluoxetine,
nitrazepam, glibenclamide, and cromakalim
by both forced swimming test (FST) and tail
suspension test (TST). Animals were
injected by various doses of nitrazepam
(0.05, 0.1, and 0.5 mg/kg). Nitrazepam at
dose of 0.5 mg/kg significantly decreased
the immobility time compared to control
group in both FST and TST. Fluoxetine also
showed such a response. Co-administration
of nitrazepam (0.05 mg/kg) with
glibenclamide in TST (1 mg/kg) and in FST
(0.3, 1 mg/kg) also showed antidepressantlike response. Beside, cromakalim (0.1
mg/kg) could reverse the antidepressant-like
effect of nitrazepam (0.5 mg/kg) in both
FST and TST, while cromakalim
and glibenclamide alone could not change
the immobility time compared to control
group (P40.05). The hole-board test revealed
that nitrazepam at doses of 0.5 and 0.1
mg/kg could increase the activity of the
animal’s head-dipping and boost the
curiosity and exploration behavior of mice.The results of this study revealed that
nitrazepam may possess antidepressant-like
properties and this effect is dependent to
potassium channels in both FST and TST. |